Team:Newcastle/3 September 2010

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Contents

yneA

Aims

The aim of the experiment is to test for the correct integration of yneA in pGFPrrnb plasmid.

Materials and protocols

  1. Please refer to: Plasmid extraction.
  2. Please refer to:Restriction digest. We used Nhe1 and Spe1 to remove the yneA from pGFP-rrnB.
  3. Please refer to: Gel electrophoresis for running all the digested plasmid fragments.

Results

03.09.10.png

Conclusion

The results show that the digest works, there is a band at approximately 541bp corresponding to yneA and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.


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