Team:Newcastle/3 September 2010

From 2010.igem.org

Revision as of 14:18, 27 October 2010 by Swoodhouse (Talk | contribs)

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

yneA

Aims

The aim of the experiment is to test if the filamentous cell part was cloned into pGFP-rrnB successfully.

Materials and protocols

  1. Please refer to: Plasmid extraction.
  2. Please refer to:Restriction digest. We used Nhe1 and Spe1 to remove the yneA from pGFP-rrnB.
  3. Please refer to: Gel electrophoresis for running all the digested plasmid fragments.

Results

03.09.10.png

Figure 1: Gel electrophoresis result for restriction digest of pGFPrrnB and yneA with Nhe1 and Spe1.

  • Lane 1: 1kb DNA ladder
  • Lane 2: Tube 1
  • Lane 3: Tube 2
  • Lane 4: Tube 3
  • Lane 5: Tube 4
  • Lane 6: Tube 5
  • Lane 7: Tube 6
  • Lane 8: Tube 7
  • Lane 9: Tube 8
  • Lane 10: Tube 9
  • Lane 11: Tube 10
  • Lane 12: Tube 11
  • Lane 13: Tube 12
  • Lane 14: 1kb DNA ladder

Conclusion

The results show that the digest works, there is a band at approximately 541bp corresponding to yneA and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2, 3, 4, 6, 7, 8, 9, 11 and 12.


Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon