Team:Newcastle/3 September 2010

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(Difference between revisions)
(Materials and protocols)
(Materials and protocols)
Line 9: Line 9:
#Please refer to: [[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction| Plasmid extraction]].
#Please refer to: [[Team:Newcastle/Qiagen_Minipreps#Plasmid_extraction| Plasmid extraction]].
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#Please refer to:[[Team:Newcastle/Restriction_digests|Restriction digest]]. We used EcoR1 to linearise the plasmid so that we acn run it on the gel.
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#Please refer to:[[Team:Newcastle/Restriction_digests|Restriction digest]]. We used Nhe1 and Spe1 to remove the ''yneA'' from pGFP-rrnB.  
#Please refer to:[[Team:Newcastle/Gel_electrophoresis| Gel electrophoresis]] for running all the digested plasmid fragments.
#Please refer to:[[Team:Newcastle/Gel_electrophoresis| Gel electrophoresis]] for running all the digested plasmid fragments.

Revision as of 23:50, 25 October 2010

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Contents

yneA

Aims

The aim of the experiment is to test for the correct integration of yneA in pGFPrrnb plasmid.

Materials and protocols

  1. Please refer to: Plasmid extraction.
  2. Please refer to:Restriction digest. We used Nhe1 and Spe1 to remove the yneA from pGFP-rrnB.
  3. Please refer to: Gel electrophoresis for running all the digested plasmid fragments.

Results

YneAGFPrrnb.jpeg

Conclusion

The results show that the digest works, there is a band at 541bp corresponding to yneA and a band at 8.4kbp corresponding to GFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.


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