Team:Newcastle/27 August 2010

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(Difference between revisions)
(Materials and Protocol)
(Results)
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==Results==
==Results==
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[[Image:Newcastle_controlgel_270810.jpg|500px]]
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[[Image:Newcastle_controlgel_270810.jpg|500px|centre]]
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'''Figure 1''': Gel electrophoresis of the amplified PCR products and restriction digest
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*'''Lane 1''': 1 Kb ladder
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*'''Lane 2''': pSB1C3 (for Subtilin Immunity)
==Discussion==
==Discussion==

Revision as of 10:37, 27 August 2010

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Contents

PCR and digestion controls

Aims

Since we have recently had problems with both PCR and restriction digests, we set up positive controls to ensure that there is nothing wrong with any of our enzymes or ...

Materials and Protocols

Please refer to the restriction digest, Phusion PCR and gel electrophoresis protocols.

...

Results

Newcastle controlgel 270810.jpg

Figure 1: Gel electrophoresis of the amplified PCR products and restriction digest

  • Lane 1: 1 Kb ladder
  • Lane 2: pSB1C3 (for Subtilin Immunity)

Discussion

Worked correctly.. nothing is wrong with our enzymes.. in the case of our rocF plasmid PCRs, it must be the primers which are the problem.. new ones are supposed to be arriving today.. in the other cases, repeat..

yneA

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