Revision as of 20:01, 21 October 2010

Polymerase Chain Reaction

To amplify a desired DNA fragment by using a set of appropriate primers flanking the fragment.

Please refer to: PCR for materials required and protocol.

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 {{Team:Newcastle/mainbanner}}
-'''18 June 2010'''
 =Polymerase Chain Reaction=
-==Protocol==
-Polymerase Chain Reaction (PCR) is a technique used to amplify a single or few a copies of a piece of DNA generating thousands to millions of copies of a particular DNA sequence.
+==Aim==
-# To avoid contamination, wear gloves
-# To achieve optimum results, always do everything on ice
+To amplify a desired DNA fragment by using a set of appropriate primers flanking the fragment.
+==Materials and Protocol==
+Please refer to: [[Team:Newcastle/PCR| PCR]] for materials required and protocol.
-A basic PCR set up requires several components and reagents.These components include:
-#DNA template that contains the DNA region (target) to be amplified.
-#Two primers that are complementary to the 3' (three prime) ends of each of the sense and anti-sense strand of the DNA target.
-#Taq polymerase or another DNA polymerase with a temperature optimum at around 70 °C.
-#Deoxynucleoside triphosphates, the building blocks from which the DNA polymerases synthesizes a new DNA strand.
-#Buffer solution, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.
 [[Image:fri-gel.png|400px|center]]