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From 2010.igem.org

Revision as of 10:30, 19 July 2010 (view source) Yessa (Talk | contribs) (→Summary) ← Older edit		Revision as of 10:50, 19 July 2010 (view source) Yessa (Talk | contribs) (→Summary) Newer edit →
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	==Summary==		==Summary==
-	Screening for insert	+	Screening for lacI insert:
	#Miniprep		#Miniprep
	#Restriction digests		#Restriction digests

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Revision as of 10:50, 19 July 2010

Summary

Screening for lacI insert:

1. Miniprep
2. Restriction digests
   • Single digest with Pst1
   • double digests with Pst1 and Xba1
3. Gel electrophoresis

Set up liquid broth culture in LB