Team:Newcastle/12 August 2010

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=Subtilin Immunity BioBrick=
 
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==Aims==
 
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Subtilin is required as a cell-signalling molecule in order to trigger calcium carbonate precipitation, as well as bacteria filament formation. We aim to produce 2 subtilin BioBricks; Production and Immunity. Because subtilin is a lantibiotic that is found naturally in ''Bacillus subtilis'' ATCC 6633, the introduction of subtilin into our ''Bacillus'' 168 cells would actually kill them. Therefore, it is essential to establish immunity for 168 strains.
 
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==Materials and Protocol==
 
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Please refer to the [[Team:Newcastle/PCR#Phusion_PCR| Phusion PCR]] protocol.
 
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{{Team:Newcastle/footer}}
 

Revision as of 11:40, 10 August 2010

Retrieved from "http://2010.igem.org/Team:Newcastle/12_August_2010"