Team:Newcastle/11 August 2010

From 2010.igem.org

(Difference between revisions)
(Subtilin Immunity BioBrick)
(Materials and Protocol)
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==Materials and Protocol==
==Materials and Protocol==
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Please refer to [[TeamNewcastleTransformation_of_E._coli|transformation of E.coli]].
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Please refer to [[TeamNewcastleTransformation_of_E._coli|transformation of ''E. coli'']].

Revision as of 07:08, 12 August 2010

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Contents

Gibson cloning of the rocF BioBrick

Aim

The aim of this experiment is to assemble the fragments we extracted yesterday in order to construct the rocF BioBrick and clone it into pSB1C3 in one step.

Subtilin Immunity BioBrick

Aims

Our aims for today are to run the gel electrophoresis to check whether we have the correct fragement sizes on the four parts that are we amplified yesterday. If the PCR worked, we will then perform gel extraction and then perform another gel electrophoresis for the extracted gel in order to obtain our BioBrick parts.

Newcastle Loaded Gel for Subtilin Immunity BioBrick.JPG

Materials and protocol

Please refer to the gel electrophoresis and the gel extraction protocols.

Results

Figure #: Gel electrophoresis of the PCR products of the parts required for the subtilin immunity BioBrick.

  • Lane 1: 1 kb DNA ladder
  • Lane 2: Plasmid Vector (pSB1C3)
  • Lane 3: Promoter and RBS (pVeg-SpoVG)
  • Lane 4: spaIFEG Gene Cluster
  • Lane 5: Double terminator
  • Lane 6: 100 bp DNA ladder

Transformation of hyperspank and spoVG

Aim

To transform competent E.coli DH5α with hyperspank and spoVG.

Materials and Protocol

Please refer to transformation of E. coli.


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