Team:Newcastle/10 September 2010

From 2010.igem.org

(Difference between revisions)
(Miniprep of BBa_I13401 (GFP coding sequence + terminator))
(Double digests)
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==Double digests==
==Double digests==
 +
.. double digests for standard BioBrick assembly.. cut the arabinose-inducible promoter BioBrick with EcoRI and SpeI, cut gfp+terminator BioBrick with EcoRI and XbaI, for later ligation..
==Gel extraction of digests==
==Gel extraction of digests==

Revision as of 10:23, 10 September 2010

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Contents

Arabinose-inducible promoter characterisation

...

Miniprep of BBa_I13401 (GFP coding sequence + terminator)

..from last night's overnight cultures from the 8th of september transformation.

Double digests

.. double digests for standard BioBrick assembly.. cut the arabinose-inducible promoter BioBrick with EcoRI and SpeI, cut gfp+terminator BioBrick with EcoRI and XbaI, for later ligation..

Gel extraction of digests

Ligation

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