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NT Cell Transformation Results

Plated NT Cells Immediately after Transformation

RD29A+RFP Transformed NT Cells after about 3 weeks of growth: The NT Cells shown above were transformed with RD29A+RFP, a composite part in which the RD29A promoter is turned on due to external stimuli such as cold, drought or salinety. When the RD29A promoter is turned on, it turned on a reporter such as RFP. After about 3 weeks of NT cell growth, the transformed cells were "cold stressed" for about 3hrs in 4 degrees Celsius. The results shown indicate that when cold stressed,the transformed NT cells become fluorescent with RFP.