"
Team:Nevada/Interpolated Data
From 2010.igem.org
Contents |
Interpolation Method
Natural cubic splines were made based on the four time points for each gene. This produced 3 splines per gene: one for the interval from 0 to 3 hours, the second from 3 to 6 hours and a final one for 6 to 24 hours. For all genes, the final spline was also used to extrapolate out to 30 hours, in order to get a sense of whether or not some of these genes may have a delayed response to freezing. 16 time points were taken from the 30-hour interpolated/extrapolated data at 2 hour intervals to produce this data set, i.e. ( t0 = 0 hours, t1 = 2 hours, t2 = 4 hours...t15 = 30 hours ).
Interpolated Data Set
| Raw Data | ICE1 | DREB1A | DREB1B | DREB1C | rd29A | ZAT6 | RAP2.1 | RAP2.6 |
| t0 | 1358.1 | 96.6 | 24.5 | 69.4 | 1509.2 | 145.3 | 100.4 | 58.5 |
| t1 | 1450.6 | 4600.5 | 3748.3 | 9051.6 | 2125.8 | 455.4 | 78.4 | 58.2 |
| t2 | 1515 | 6617.4 | 5072.8 | 12724.1 | 5330.4 | 965.3 | 166.6 | 55.8 |
| t3 | 1547.9 | 6181.2 | 4099 | 11249.3 | 10644.4 | 1620.6 | 357.8 | 52.3 |
| t4 | 1566.7 | 5400.5 | 2970.9 | 9273.8 | 15161.9 | 2168.8 | 549.6 | 51.6 |
| t5 | 1576.1 | 4709.9 | 2112.2 | 7731.3 | 18402.6 | 2571.8 | 722.5 | 53.9 |
| t6 | 1577.2 | 4098.1 | 1489.2 | 6567.6 | 20526.3 | 2847.7 | 878.6 | 59 |
| t7 | 1571.2 | 3554 | 1068.2 | 5728.6 | 21692.4 | 3014.6 | 1020.5 | 66.4 |
| t8 | 1559.4 | 3066.1 | 815.6 | 5160.2 | 22060.6 | 3090.8 | 1150.5 | 75.8 |
| t9 | 1542.8 | 2623.4 | 697.7 | 4808.4 | 21790.5 | 3094.3 | 1271.1 | 86.8 |
| t10 | 1522.6 | 2214.4 | 680.8 | 4618.8 | 21041.6 | 3043.4 | 1384.4 | 98.9 |
| t11 | 1500.2 | 1828 | 731.3 | 4537.6 | 19973.6 | 2956.1 | 1493.7 | 111.8 |
| t12 | 1476.5 | 1452.8 | 815.4 | 4510.4 | 18745.9 | 2850.7 | 1599.3 | 125 |
| t13 | 1452.8 | 1077.6 | 899.5 | 4483.2 | 17518.2 | 2745.3 | 1705.5 | 138.3 |
| t14 | 1430.4 | 691.2 | 950 | 4402 | 16450.2 | 2658 | 1814.2 | 151.1 |
| t15 | 1410.2 | 282.2 | 933.1 | 4212.4 | 15701.3 | 2607.1 | 1927.5 | 163.2 |
Log Base 2 of Normalized Ratios
| Log2 Values | ICE1 | DREB1A | DREB1B | DREB1C | rd29A | ZAT6 | RAP2.1 | RAP2.6 |
| t0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| t1 | 0.0951 | 5.5736 | 7.2573 | 7.0271 | 0.4942 | 1.6481 | -0.3568 | -0.0074 |
| t2 | 0.1577 | 6.0981 | 7.6939 | 7.5184 | 1.8205 | 2.7319 | 0.7306 | -0.0682 |
| t3 | 0.1887 | 5.9997 | 7.3863 | 7.3407 | 2.8182 | 3.4794 | 1.8334 | -0.1616 |
| t4 | 0.2061 | 5.8049 | 6.9220 | 7.0621 | 3.3286 | 3.8998 | 2.4526 | -0.1811 |
| t5 | 0.2148 | 5.6075 | 6.4298 | 6.7996 | 3.6081 | 4.1457 | 2.8472 | -0.1182 |
| t6 | 0.2158 | 5.4068 | 5.9256 | 6.5643 | 3.7656 | 4.2927 | 3.1294 | 0.0123 |
| t7 | 0.2103 | 5.2013 | 5.4463 | 6.3671 | 3.8453 | 4.3749 | 3.3454 | 0.1827 |
| t8 | 0.1994 | 4.9882 | 5.0570 | 6.2163 | 3.8696 | 4.4109 | 3.5184 | 0.3738 |
| t9 | 0.1840 | 4.7633 | 4.8318 | 6.1145 | 3.8518 | 4.4125 | 3.6622 | 0.5693 |
| t10 | 0.1649 | 4.5187 | 4.7964 | 6.0564 | 3.8014 | 4.3886 | 3.7854 | 0.7575 |
| t11 | 0.1436 | 4.2421 | 4.8996 | 6.0308 | 3.7262 | 4.3466 | 3.8951 | 0.9344 |
| t12 | 0.1206 | 3.9107 | 5.0567 | 6.0222 | 3.6347 | 4.2942 | 3.9936 | 1.0954 |
| t13 | 0.0972 | 3.4797 | 5.1983 | 6.0134 | 3.5370 | 4.2399 | 4.0864 | 1.2413 |
| t14 | 0.0748 | 2.8390 | 5.2771 | 5.9871 | 3.4462 | 4.1932 | 4.1755 | 1.3690 |
| t15 | 0.0543 | 1.5466 | 5.2512 | 5.9236 | 3.3790 | 4.1653 | 4.2629 | 1.4801 |
Boolean Values (Threshold of 3.5)
| Boolean Values | ICE1 | DREB1A | DREB1B | DREB1C | RD29A | ZAT6 | RAP2.1 | RAP2.6 |
| t0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| t1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
| t2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
| t3 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
| t4 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 |
| t5 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 |
| t6 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 |
| t7 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 |
| t8 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 |
| t9 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 |
| t10 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 |
| t11 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 |
| t12 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 0 |
| t13 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 |
| t14 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 |
| t15 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 |
Boolean Input Functions
- Link to ICE1 inputs
- Link to DREB1B inputs
- Link to DREB1C inputs
- Link to rd29A inputs
- Link to RAP2.6 inputs
