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Team:Nevada/Agrobacterium Transformations
From 2010.igem.org
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| + | '''''Agrobacterium tumefaciens'' Mediated Transformation of Plant Cells''' | ||
| + | <br> | ||
| + | ''Agrobacterium tumefaciens'' | ||
| + | *Gram (-) soil bacterium | ||
| + | *Causative agent in crown gall disease | ||
| + | *Disease is the result of interkingdom transfer of DNA (i.e. bacteria --> plant) | ||
| + | <br> | ||
| + | '''Molecular Progression of Agrobacterium Infection''' | ||
| + | <br>1. chemical perception of host | ||
| + | <br>2. induction of virulence genes | ||
| + | <br>3. formation of infection apparatus | ||
| + | <br>4. preparation of T-DNA | ||
| + | <br>5. export T-DNA to plant cell | ||
| + | <br>6. import T-DNA to plant nucleus | ||
| + | <br>7. integration of T-DNA into host genome | ||
| + | <br>8. expression of T-DNA | ||
| + | <br> | ||
| + | <br> | ||
| + | <html><a href="https://static.igem.org/mediawiki/2010/d/d0/Agro_theory.png"><img src="https://static.igem.org/mediawiki/2010/d/d0/Agro_theory.png" class="shadow" style="float:left;width:800px;margin:10px"></a> | ||
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Revision as of 02:43, 26 October 2010
Agrobacterium Transformations
Agrobacterium tumefaciens Mediated Transformation of Plant Cells
Agrobacterium tumefaciens
- Gram (-) soil bacterium
- Causative agent in crown gall disease
- Disease is the result of interkingdom transfer of DNA (i.e. bacteria --> plant)
Molecular Progression of Agrobacterium Infection
1. chemical perception of host
2. induction of virulence genes
3. formation of infection apparatus
4. preparation of T-DNA
5. export T-DNA to plant cell
6. import T-DNA to plant nucleus
7. integration of T-DNA into host genome
8. expression of T-DNA
