http://2010.igem.org/wiki/index.php?title=Team:Nevada&feed=atom&action=historyTeam:Nevada - Revision history2024-03-29T06:45:10ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Nevada&diff=199122&oldid=prevMpolasko at 22:40, 27 October 20102010-10-27T22:40:46Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Abstract ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Abstract ==</div></td></tr>
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</table>Mpolaskohttp://2010.igem.org/wiki/index.php?title=Team:Nevada&diff=198871&oldid=prevMpolasko at 22:25, 27 October 20102010-10-27T22:25:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Abstract ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Abstract ==</div></td></tr>
</table>Mpolaskohttp://2010.igem.org/wiki/index.php?title=Team:Nevada&diff=198711&oldid=prevMpolasko at 22:19, 27 October 20102010-10-27T22:19:14Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Abstract ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Abstract ==</div></td></tr>
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</table>Mpolaskohttp://2010.igem.org/wiki/index.php?title=Team:Nevada&diff=196275&oldid=prevEbersaba at 20:57, 27 October 20102010-10-27T20:57:48Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Thank you to <span style="font-weight:bold;">[http://www.promega.com/Catalog/CountrySelect.aspx?returnurl=/Default.asp Promega Co.]</span> for free enzyme donations.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Thank you to <span style="font-weight:bold;">[http://www.promega.com/Catalog/CountrySelect.aspx?returnurl=/Default.asp Promega Co.]</span> for free enzyme donations.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Thank you to <span style="font-weight:bold;">[http://www.invitrogen.com/site/us/en/home.html?cid=covinvggl89100000002336s& Invitrogen Co.]</span> for a discount on our Vector NTI program.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Thank you to <span style="font-weight:bold;">[http://www.invitrogen.com/site/us/en/home.html?cid=covinvggl89100000002336s& Invitrogen Co.]</span> for a discount on our Vector NTI program.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Thank you to the <span style="font-weight:bold;">[http://sparks22.adventistchurchconnect.org/ Sparks Seventh-day Adventist Church]</span> <del class="diffchange diffchange-inline">who donated </del>$1,000 in response to the sponsorship letters.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Thank you to the <span style="font-weight:bold;">[http://sparks22.adventistchurchconnect.org/ Sparks Seventh-day Adventist Church]</span> <ins class="diffchange diffchange-inline">for the </ins>$1,000 <ins class="diffchange diffchange-inline">donation </ins>in response to the sponsorship letters.</div></td></tr>
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</table>Ebersabahttp://2010.igem.org/wiki/index.php?title=Team:Nevada&diff=193669&oldid=prevEbersaba at 19:34, 27 October 20102010-10-27T19:34:11Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Thank you to <span style="font-weight:bold;">[http://www.promega.com/Catalog/CountrySelect.aspx?returnurl=/Default.asp Promega Co.]</span> for free enzyme donations.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Thank you to <span style="font-weight:bold;">[http://www.promega.com/Catalog/CountrySelect.aspx?returnurl=/Default.asp Promega Co.]</span> for free enzyme donations.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Thank you to <span style="font-weight:bold;">[http://www.invitrogen.com/site/us/en/home.html?cid=covinvggl89100000002336s& Invitrogen Co.]</span> for a discount on our Vector NTI program.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Thank you to <span style="font-weight:bold;">[http://www.invitrogen.com/site/us/en/home.html?cid=covinvggl89100000002336s& Invitrogen Co.]</span> for a discount on our Vector NTI program.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Thank you to the <span style="font-weight:bold;">[http://sparks22.adventistchurchconnect.org/ Sparks Seventh-day Adventist Church]</span> who donated $1,000 in response to the sponsorship letters.</ins></div></td></tr>
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</table>Ebersabahttp://2010.igem.org/wiki/index.php?title=Team:Nevada&diff=193166&oldid=prevEbersaba at 19:18, 27 October 20102010-10-27T19:18:01Z<p></p>
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</table>Ebersabahttp://2010.igem.org/wiki/index.php?title=Team:Nevada&diff=193102&oldid=prevEbersaba at 19:15, 27 October 20102010-10-27T19:15:52Z<p></p>
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</table>Ebersabahttp://2010.igem.org/wiki/index.php?title=Team:Nevada&diff=193021&oldid=prevEbersaba at 19:13, 27 October 20102010-10-27T19:13:16Z<p></p>
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</table>Ebersabahttp://2010.igem.org/wiki/index.php?title=Team:Nevada&diff=172313&oldid=prevMpolasko at 03:06, 27 October 20102010-10-27T03:06:09Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Fluorescent Plant Image Taken From: http://www.edinformatics.com/inventions_inventors/genetic_engineering.htm</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p <ins class="diffchange diffchange-inline">style="text-align:right;"><span style="font-size:10px;"</ins>>Fluorescent Plant Image Taken From: http://www.edinformatics.com/inventions_inventors/genetic_engineering.htm<ins class="diffchange diffchange-inline"></span></ins></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
</table>Mpolaskohttp://2010.igem.org/wiki/index.php?title=Team:Nevada&diff=169633&oldid=prevHilarya: /* Abstract */2010-10-27T00:27:22Z<p><span class="autocomment">Abstract</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our long term goals are to develop plant biosensors that reliably measure changes in the local environment. In the process of developing biosensors, the''' 2010 Nevada iGEM Team''' need to develop fundamental promoters, reporters, and plasmids for plants. Therefore, the''' 2010 Nevada iGEM Team''' has three goals for this year’s competition. First, we are going to test the validity of utilizing Nicotiana tabacum (NT cells) as a model for the expression of higher plant genes for future iGEM competitions. NT cells are a faster, cheaper, and safer model than traditional plant transformation. These cells can therefore be utilized as a quick proof-of-concept test model before moving synthetic constructs into plants of interest. We also aim to produce an iGEM-compatible plant-specific plasmid, several stress-inducible plant promoters, reporter genes containing Kozak sequences (ribosome binding sites) and terminators that conform to BioBrick standards. Lastly, we hope to measure the induction of these stress promoters in real-time by performing a fluorometry assay in which stress will be applied to NT cells and fluorescent output by a reporter (GFP) will be measured to detail induction in real time. This method has a distinct advantage over microarrays since microarrays are only one ‘snapshot’ in time.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our long term goals are to develop plant biosensors that reliably measure changes in the local environment. In the process of developing biosensors, the''' 2010 Nevada iGEM Team''' need to develop fundamental promoters, reporters, and plasmids for plants. Therefore, the''' 2010 Nevada iGEM Team''' has three goals for this year’s competition. First, we are going to test the validity of utilizing Nicotiana tabacum (NT cells) as a model for the expression of higher plant genes for future iGEM competitions. NT cells are a faster, cheaper, and safer model than traditional plant transformation. These cells can therefore be utilized as a quick proof-of-concept test model before moving synthetic constructs into plants of interest. We also aim to produce an iGEM-compatible plant-specific plasmid, several stress-inducible plant promoters, reporter genes containing Kozak sequences (ribosome binding sites) and terminators that conform to BioBrick standards. Lastly, we hope to measure the induction of these stress promoters in real-time by performing a fluorometry assay in which stress will be applied to NT cells and fluorescent output by a reporter (GFP) will be measured to detail induction in real time. This method has a distinct advantage over microarrays since microarrays are only one ‘snapshot’ in time.</p></div></td></tr>
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</table>Hilarya