Team:Monash Australia/Notebook

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=== 09/08/10 ===
=== 09/08/10 ===
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Created 1L SOB media using Tom Knights protocol with modifications as followed:
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# 5g yeast extract
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# 0.5g NaCl
 +
# 0.19g KCl
 +
# 20g Tryptone
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of the 1L of SOB media, 2x 250ml was autoclaved; one was used to make agar medium the other was used for medium. The other 500ml was left for storage, however was subsequently destroyed due to contamination.
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3.75g of agar was added to 250ml of SOB media to produce agar media
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Created a CCMB80 Buffer as followed:
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# 2.5ml KoAc
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# 2.95g CaCl2.2H2O
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# 1g MnCl2.4H2O
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# 0.5g MgCl2.6H2O
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# 25ml Glycerol
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# HCl to bring pH ~ 6.4
===Date===
===Date===

Revision as of 07:58, 20 August 2010


Contents

Lab Notebook

09/08/10

Created 1L SOB media using Tom Knights protocol with modifications as followed:

  1. 5g yeast extract
  2. 0.5g NaCl
  3. 0.19g KCl
  4. 20g Tryptone

of the 1L of SOB media, 2x 250ml was autoclaved; one was used to make agar medium the other was used for medium. The other 500ml was left for storage, however was subsequently destroyed due to contamination.

3.75g of agar was added to 250ml of SOB media to produce agar media

Created a CCMB80 Buffer as followed:

  1. 2.5ml KoAc
  2. 2.95g CaCl2.2H2O
  3. 1g MnCl2.4H2O
  4. 0.5g MgCl2.6H2O
  5. 25ml Glycerol
  6. HCl to bring pH ~ 6.4

Date

Date

Date

Date