Team:Michigan/Protocols

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General Outline for Biobrick Manipulations

Igem order of procedures.jpg

Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation-heat shock

This is the procedure we can perform in the ERB

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Heat shock

Transformation-electroporation

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

  • Ginko Bioworks Protocol
  • Jeremy Minty Protocol