Team:Michigan/Protocols

From 2010.igem.org

(Difference between revisions)
(Protocols)
(Protocols)
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[[Media:NanR_Cloning.pdf|NanR cloning]]
[[Media:NanR_Cloning.pdf|NanR cloning]]
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*Updated Colony PCR Protocol
[[Media:NA Extraction.pdf|NA Extraction Protocol]]
[[Media:NA Extraction.pdf|NA Extraction Protocol]]

Revision as of 20:06, 8 October 2010


Michigan Header




Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Transformation-heat shock

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Heat shock

Transformation-electroporation

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Electroporation

Electroporation Protocol 2

  • Adopted from Jeremy Minty's protocol
  • General protocol with specific instructions for ERB/Lin lab equipment

MODIFIED Miniprep

Nanodrop

DNA Digest

Digest Reagents Calculator

Running a Gel

DNA Purification

Quick Ligation Protocol

Gel extraction

Updated T4 DNA Ligase Protocol (Not quick ligase)

  • For additional information/questions, refer to [http://www.neb.com/nebecomm/tech_reference/modifying_enzymes/ligation_tips.asp NEB Ligation Tips]
  • Ligation
    • Ginko Bioworks Protocol
    • Jeremy Minty Protocol

Group-Specific Protocols

Oil Sands

Revised biofilm assay protocol

Alex's biofilm assay protocol

General primer design

Detailed primer design

colony PCR protocol

NanR cloning

  • Updated Colony PCR Protocol

NA Extraction Protocol

Pili

FimB Ligation

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols

General Outline for Biobrick Manipulations

Igem order of procedures.jpg