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From 2010.igem.org

Revision as of 18:38, 4 August 2010 (view source) Kevijose (Talk | contribs) ← Older edit		Revision as of 23:28, 4 August 2010 (view source) Infekt (Talk | contribs) (→Quorum Sensing) Newer edit →
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	[[Media:Quorum+Sensing+Tables-1-.pdf|Logic Table]]		[[Media:Quorum+Sensing+Tables-1-.pdf|Logic Table]]
-	[[Media:QS Procedures.pdf|Lsr Circuit Test Protocols]]	+	[[Media:QS_Procedures-1-.pdf|Lsr Circuit Test Protocols]]
	==General Outline for Biobrick Manipulations==		==General Outline for Biobrick Manipulations==
	[[Image:Igem_order_of_procedures.jpg|900px]]		[[Image:Igem_order_of_procedures.jpg|900px]]

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Revision as of 23:28, 4 August 2010

Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Transformation-heat shock

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

MODIFIED Miniprep

• Miniprep

Nanodrop

DNA Digest

Running a Gel

DNA Purification

Ligation

• Ginko Bioworks Protocol
• Jeremy Minty Protocol

Group-Specific Protocols

Oil Sands

biofilm assay protocol

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols

General Outline for Biobrick Manipulations