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Team:Michigan/Protocols
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[[Media:6-27-2010_P._putida_antibiotic_resistance_protocol.pdf|''P. putida'' KT2440 antibotic resistance tolerance]] | [[Media:6-27-2010_P._putida_antibiotic_resistance_protocol.pdf|''P. putida'' KT2440 antibotic resistance tolerance]] | ||
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| + | [[Media:Protocol_for_Culturing_Strains_from_CGSC.pdf|Culturing CGSC Strains]] | ||
'''DNA Manipulation''' | '''DNA Manipulation''' | ||
Revision as of 03:01, 23 July 2010
General Outline for Biobrick Manipulations
Protocols
Using Lab Equipment
Obtaining Deionized Water in the ERB
Enigeering Research Building Autoclave
Cell Culture
Making cultures from a -80C freezer stock
P. putida KT2440 antibotic resistance tolerance
DNA Manipulation
This is the procedure we can perform in the ERB
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Heat shock
Transformation-electroporation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Electroporation
- Ginko Bioworks Protocol
- Jeremy Minty Protocol
