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Revision as of 20:42, 8 July 2010 (view source) Infekt (Talk | contribs) (→Protocols) ← Older edit		Revision as of 00:06, 18 July 2010 (view source) Infekt (Talk | contribs) (→Protocols) Newer edit →
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	*Ginko Bioworks Protocol		*Ginko Bioworks Protocol
	*Jeremy Minty Protocol		*Jeremy Minty Protocol
		+	===Group-Specific Protocols===
		+	====Quorum Sensing====
		+	[[Media:Quorum+Sensing+Tables[1].pdf|Testing AI-2 Response]]

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Revision as of 00:06, 18 July 2010

General Outline for Biobrick Manipulations

Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation-heat shock

This is the procedure we can perform in the ERB

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

• Ginko Bioworks Protocol
• Jeremy Minty Protocol

Group-Specific Protocols

Quorum Sensing

[[Media:Quorum+Sensing+Tables[1].pdf|Testing AI-2 Response]]