Team:Michigan/Protocols

From 2010.igem.org

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(Protocols)
(Protocols)
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'''Using Lab Equipment'''
'''Using Lab Equipment'''
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[[Media:Protocol for Obtaining Deionized Water in the ERB]]
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[[Media:Obtaining Deionized Water in the ERB]]
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[[Media:ERB Spectrophotometer]]
[[Media:7-6-2010_ERB_Autoclave_Liquid_Sterilization_Protocol.pdf|Enigeering Research Building Autoclave]]
[[Media:7-6-2010_ERB_Autoclave_Liquid_Sterilization_Protocol.pdf|Enigeering Research Building Autoclave]]

Revision as of 20:35, 8 July 2010


Michigan Header




General Outline for Biobrick Manipulations

Igem order of procedures.jpg

Protocols

Using Lab Equipment

Media:Obtaining Deionized Water in the ERB Media:ERB Spectrophotometer Enigeering Research Building Autoclave

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation-heat shock

This is the procedure we can perform in the ERB

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Heat shock

Transformation-electroporation

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

  • Ginko Bioworks Protocol
  • Jeremy Minty Protocol