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From 2010.igem.org

Revision as of 17:51, 17 October 2010 (view source) Chillywings (Talk | contribs) (→Protocols) ← Older edit		Revision as of 23:24, 17 October 2010 (view source) Chillywings (Talk | contribs) (→Protocols) Newer edit →
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	*Adopted from Jeremy Minty's protocol		*Adopted from Jeremy Minty's protocol
	*General protocol with specific instructions for ERB/Lin lab equipment		*General protocol with specific instructions for ERB/Lin lab equipment
		+
		+	[[Media:PCR Screening Protocol.pdf|PCR Screening Protocol]]
		+	*Efficient protocol for screening transformants after a ligation
	[[Media:MODIFIED_Miniprep_Protocol.pdf|MODIFIED Miniprep]]		[[Media:MODIFIED_Miniprep_Protocol.pdf|MODIFIED Miniprep]]

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Revision as of 23:24, 17 October 2010

Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Direct Plating Transformation Protocol

• Very simple protocol
• Twice as efficient as standard heat shock

Transformation-heat shock

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Electroporation Protocol 2

• Adopted from Jeremy Minty's protocol
• General protocol with specific instructions for ERB/Lin lab equipment

PCR Screening Protocol

• Efficient protocol for screening transformants after a ligation

MODIFIED Miniprep

• Miniprep

Nanodrop

DNA Digest

Digest Reagents Calculator

Gel Electrophoresis

DNA Purification

Quick Ligation Protocol

Gel extraction

Updated T4 DNA Ligase Protocol (Not quick ligase)

• For additional information/questions, refer to [http://www.neb.com/nebecomm/tech_reference/modifying_enzymes/ligation_tips.asp NEB Ligation Tips]
• Ligation
  • Ginko Bioworks Protocol
  • Jeremy Minty Protocol

Group-Specific Protocols

Oil Sands

Revised biofilm assay protocol

• biofilm assay protocol

Alex's biofilm assay protocol

General primer design

Detailed primer design

colony PCR protocol

NanR cloning

• Updated Colony PCR Protocol

NA Extraction Protocol

Pili

FimB Ligation

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols

General Outline for Biobrick Manipulations