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Revision as of 14:38, 8 October 2010 (view source) Chillywings (Talk | contribs) (→Protocols) ← Older edit		Revision as of 20:06, 8 October 2010 (view source) Chillywings (Talk | contribs) (→Protocols) Newer edit →
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	[[Media:NanR_Cloning.pdf|NanR cloning]]		[[Media:NanR_Cloning.pdf|NanR cloning]]
		+	*Updated Colony PCR Protocol
	[[Media:NA Extraction.pdf|NA Extraction Protocol]]		[[Media:NA Extraction.pdf|NA Extraction Protocol]]

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Revision as of 20:06, 8 October 2010

Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Transformation-heat shock

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Electroporation Protocol 2

• Adopted from Jeremy Minty's protocol
• General protocol with specific instructions for ERB/Lin lab equipment

MODIFIED Miniprep

• Miniprep

Nanodrop

DNA Digest

Digest Reagents Calculator

Running a Gel

DNA Purification

Quick Ligation Protocol

Gel extraction

Updated T4 DNA Ligase Protocol (Not quick ligase)

• For additional information/questions, refer to NEB Ligation Tips
• Ligation
  • Ginko Bioworks Protocol
  • Jeremy Minty Protocol

Group-Specific Protocols

Oil Sands

Revised biofilm assay protocol

• biofilm assay protocol

Alex's biofilm assay protocol

General primer design

Detailed primer design

colony PCR protocol

NanR cloning

• Updated Colony PCR Protocol

NA Extraction Protocol

Pili

FimB Ligation

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols

General Outline for Biobrick Manipulations