Team:Michigan/Protocols

From 2010.igem.org

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[[Media:7-31-2010_Ag43_flu_gene_WITH_MODIFIED_PRIMER_SITES.pdf|Detailed primer design]]
[[Media:7-31-2010_Ag43_flu_gene_WITH_MODIFIED_PRIMER_SITES.pdf|Detailed primer design]]
[[Media:8-4-2010_Colony_PCR_flu.pdf|colony PCR protocol]]
[[Media:8-4-2010_Colony_PCR_flu.pdf|colony PCR protocol]]
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====Pili====
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[[Media:FimB_Ligation_Protocol.pdf|FimB Ligation]]
====Quorum Sensing====
====Quorum Sensing====

Revision as of 22:43, 23 August 2010


Michigan Header




Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Transformation-heat shock

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Heat shock

Transformation-electroporation

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Electroporation

MODIFIED Miniprep

Nanodrop

DNA Digest

Running a Gel

DNA Purification

Gel extraction

Updated T4 DNA Ligase Protocol (Not quick ligase)

  • Ligation
    • Ginko Bioworks Protocol
    • Jeremy Minty Protocol

Group-Specific Protocols

Oil Sands

Revised biofilm assay protocol

General primer design

Detailed primer design colony PCR protocol

Pili

FimB Ligation

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols

General Outline for Biobrick Manipulations

Igem order of procedures.jpg