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	[[Media:Protocol_for_DNA_Purification.pdf|DNA Purification]]		[[Media:Protocol_for_DNA_Purification.pdf|DNA Purification]]
-	[[Media:|Gel extraction]]	+	[[Media:8-8-2010_Protocol_for_gel_extraction.pdf|Gel extraction]]
	[[Media:Ligation_Protocol.pdf|Ligation]]		[[Media:Ligation_Protocol.pdf|Ligation]]

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Revision as of 18:15, 9 August 2010

Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Transformation-heat shock

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

MODIFIED Miniprep

• Miniprep

Nanodrop

DNA Digest

Running a Gel

DNA Purification

Gel extraction

Ligation

• Ginko Bioworks Protocol
• Jeremy Minty Protocol

Group-Specific Protocols

Oil Sands

biofilm assay protocol

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols

General Outline for Biobrick Manipulations