Team:Michigan/Project
From 2010.igem.org
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[[Image:Michigan-PiliReg.png|300px|thumb|left|Fig. 3 The recombinases FimB and FimE regulate the fim operon via an invertible segment of DNA.]] | [[Image:Michigan-PiliReg.png|300px|thumb|left|Fig. 3 The recombinases FimB and FimE regulate the fim operon via an invertible segment of DNA.]] | ||
[[Image:Michigan-PiliReg2.png|200px|thumb|left|Fig. 4 A model that illustrates the different states of the regulatory system.]] | [[Image:Michigan-PiliReg2.png|200px|thumb|left|Fig. 4 A model that illustrates the different states of the regulatory system.]] | ||
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+ | ===Pili Results=== | ||
+ | We were able to put FimB onto pBAD, an arabinose inducible plasmid. This should have resulted in hyperpiliated E. coli, however we were not able to find many pili on our cells when we used an electron microscope. This is discouraging, but it's not a strong indication of failure because our growth conditions may not have been ideal for pili formation. More tests should be done to determine the optimal pili growth conditions. | ||
+ | [[Image: OD_assay_all_samples.png]] | ||
+ | After conducting several assays to determine the effect of hyperpiliated E. coli on the flocculation of algae, we were not able to see very significant results. Hyperpiliated E. coli seem to flocculate the algae faster than nonpiliated E. coli, but the algae seemed to flocculate by itself. This is possibly due to contaminants in the algae. | ||
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Revision as of 23:00, 25 October 2010