Team:Michigan/Pili October
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Performed transformation of FimB into competent cells. Made plates of 1:1 and 1:3 dilutions. | Performed transformation of FimB into competent cells. Made plates of 1:1 and 1:3 dilutions. | ||
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+ | ==10/19/10== | ||
+ | ''Kevin'' | ||
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+ | Ran another OD assay to measure rate of flocculation. One key improvement in this assay is that I was able to run triplicates of each sample, to get a more accurate reading. I also started collecting data 3 hrs after inducing with arabinose, to give the cells time to grow pili. All cultures used contained the pBAD-FimB plasmid, although not all were induced. | ||
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+ | [[Media: OD_assay_data_10_19.xls|Raw Data]] | ||
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+ | [[Image: OD_assay_all_samples.png]] | ||
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+ | [[Image: OD_assay_FimB.png]] | ||
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+ | [[Image: OD_assay_K12.png]] | ||
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+ | After discussing the results, we feel as though this data does not show significant difference in the strains during the measured time period. |
Revision as of 22:29, 21 October 2010