Team:Michigan/Pili October
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| [[Team:Michigan/Pili_October#10/20/2010|10/20/2010]] | | [[Team:Michigan/Pili_October#10/20/2010|10/20/2010]] | ||
| [[Team:Michigan/Pili_October#10/21/2010|10/21/2010]] | | [[Team:Michigan/Pili_October#10/21/2010|10/21/2010]] | ||
- | | | + | | [[Team:Michigan/Pili_October#10/22/2010|10/22/2010]] |
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+ | [[Team:Michigan/Pili_Expression | Back to Pili Main Notebook Page]] | ||
==10/2/2010== | ==10/2/2010== | ||
''Kevin'' | ''Kevin'' | ||
- | Prepared tubes with K12 and ΔFim (Both w/insert) for an agglutination assay. We added algae and arabinose at an OD600 of 0.8 and then let the tubes grow | + | Prepared tubes with K12 and ΔFim (Both w/insert) for an agglutination assay. We added algae and arabinose at an OD600 of 0.8 and then let the tubes grow. |
==10/3/2010== | ==10/3/2010== | ||
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Performed transformation of FimB into competent cells. Made plates of 1:1 and 1:3 dilutions. | Performed transformation of FimB into competent cells. Made plates of 1:1 and 1:3 dilutions. | ||
- | ==10/19/ | + | ==10/19/2010== |
''Kevin'' | ''Kevin'' | ||
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[[Image: OD_assay_K12.png]] | [[Image: OD_assay_K12.png]] | ||
- | After discussing the results, we feel as though this data does not show significant difference in the strains during the measured time period. | + | After discussing the results, we feel as though this data does not show significant difference in the strains during the measured time period. The difference in the rate of OD change for the uninduced and induced strains is not particularly large, and is within standard error. In the future, we would like to run more controls. Positive controls could include strains that are guaranteed to produce pili, while negative controls would be strains that do not produce pili. We also want to discover if there are contaminants that cause the algae to flocculate by itself. |
- | ==10/ | + | ==10/20/2010== |
+ | ''Kevin'' | ||
+ | |||
+ | Used an electron microscope in the Natural Science Building in an attempt to search for pili. Unfortunately we were not able to see any, but we did find a strange exo-polysaccharide around some of our strains. We will run more strains under the EM (including some positive controls) | ||
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+ | [[Image: algae_bacteria_1200x.png |thumb|left|300px| K12 w/plasmid next to algae (1200x). An EPS can be seen attaching the bacteria to the algae. ]] | ||
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+ | [[Image: FimB_20000x.png |thumb|left|300px| FimB knockout strain at 20000x.]] | ||
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+ | <br style="clear:both"> | ||
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+ | ==10/21/2010== | ||
''Kevin'' | ''Kevin'' | ||
Grew cultures in 2095 Nat. Sci. in preparation of further EM pictures. | Grew cultures in 2095 Nat. Sci. in preparation of further EM pictures. | ||
- | + | ==10/22/2010== | |
+ | ''Kevin'' | ||
+ | |||
+ | Marcus used the EM today and collected more pictures. | ||
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+ | [[Image:FimB_plasmid_EM.png | thumb | 300px |left|FimB- w/pBAD-FimB, 17500x. An EPS can be seen.]] | ||
+ | |||
+ | [[Image:FimE_EM.png | thumb | 300px |left| FimE-, 17500x]] |
Latest revision as of 01:45, 27 October 2010