Team:Michigan/Pili June July
From 2010.igem.org
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(New page: {{Michigan Header}} {|style="color:#1c2bf2;background-color:#fafa19;font-size:9pt;text-align:center" cellpadding="5" cellspacing="0" border="1" bordercolor="#fff" width="62%" |- ! |Sunday...) |
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==6/28/2010== | ==6/28/2010== | ||
+ | ''Kevin, Marc'' | ||
+ | |||
Made a 500 mL batch of LB broth | Made a 500 mL batch of LB broth | ||
*When pouring in distilled water, pour a few mL at a time to avoid clumping. | *When pouring in distilled water, pour a few mL at a time to avoid clumping. | ||
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==6/29/2010== | ==6/29/2010== | ||
+ | ''Kevin, Marc, Alena'' | ||
+ | |||
Started growing E. coli K12 cultures | Started growing E. coli K12 cultures | ||
*Poured 2 mL of LB broth into a Falcon tube | *Poured 2 mL of LB broth into a Falcon tube | ||
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==6/30/2010== | ==6/30/2010== | ||
+ | ''Kevin, Marc'' | ||
+ | |||
Cryopreserved stock of K12 | Cryopreserved stock of K12 | ||
*1 mL located in the iGEM box in the Lin lab. | *1 mL located in the iGEM box in the Lin lab. | ||
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==7/1/2010== | ==7/1/2010== | ||
+ | ''Kevin, Marc, Alena'' | ||
+ | |||
Cryopreserved DH5α according to protocol procedure on 6/30/2010 | Cryopreserved DH5α according to protocol procedure on 6/30/2010 | ||
*Put 1 stock in -20°C fridge in 1239 | *Put 1 stock in -20°C fridge in 1239 | ||
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==7/7/2010== | ==7/7/2010== | ||
+ | ''Alena'' | ||
+ | |||
Obtain genomic DNA of CFT073 E. coli strain from Dr. Mobley's Lab | Obtain genomic DNA of CFT073 E. coli strain from Dr. Mobley's Lab | ||
*stored in -20°C fridge on ice | *stored in -20°C fridge on ice |
Revision as of 05:08, 19 October 2010