Team:Michigan/Pili June July
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+ | [[Team:Michigan/Pili_Expression | Back to Pili Main Page. ]] | ||
==6/28/2010== | ==6/28/2010== | ||
''Kevin, Marc'' | ''Kevin, Marc'' | ||
- | Made a 500 mL batch of LB broth | + | Made a 500 mL batch of LB broth. |
- | *When pouring in distilled water, pour a few mL at a time to avoid clumping. | + | *When pouring in distilled water, pour the powder a few mL at a time to avoid clumping. |
- | *It takes 20g of powder to make 1 L of broth | + | *It takes 20g of powder to make 1 L of broth. |
- | Sterilized broth using the autoclave | + | Sterilized broth using the autoclave. |
- | *The temperature setting on the autoclave is off by a little bit | + | *The temperature setting on the autoclave is off by a little bit. |
*Set dial 2 notches below 134°C. | *Set dial 2 notches below 134°C. | ||
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''Kevin, Marc, Alena'' | ''Kevin, Marc, Alena'' | ||
- | Started growing E. coli K12 cultures | + | Started growing E. coli K12 cultures: |
*Poured 2 mL of LB broth into a Falcon tube | *Poured 2 mL of LB broth into a Falcon tube | ||
*Used strain of K12 from Dr. Lin's freezer | *Used strain of K12 from Dr. Lin's freezer | ||
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''Kevin, Marc'' | ''Kevin, Marc'' | ||
- | Cryopreserved stock of K12 | + | Cryopreserved stock of K12: |
*1 mL located in the iGEM box in the Lin lab. | *1 mL located in the iGEM box in the Lin lab. | ||
*1 mL located in the lab freezer. | *1 mL located in the lab freezer. | ||
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''Alena'' | ''Alena'' | ||
- | + | Obtained genomic DNA of CFT073 E. coli strain from Dr. Mobley's Lab | |
*stored in -20°C fridge on ice | *stored in -20°C fridge on ice | ||
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In order to test how effectively the pili flocculate, we are planning to create an E. coli strain with fimE knocked out. | In order to test how effectively the pili flocculate, we are planning to create an E. coli strain with fimE knocked out. | ||
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+ | [[#top | Back to top.]] |
Latest revision as of 01:15, 27 October 2010