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From 2010.igem.org

	Sunday	Monday	Tuesday	Wednesday	Thursday	Friday	Saturday
Week 1	-	6/28/2010	6/29/2010	6/30/2010	7/1/2010	-	-
Week 2	-	-	-	7/7/2010	-	-	-
Week 3	-	-	-	-	-	-	-

Pili Expression Team

This team includes Marc Singer, Kevin Joseph, and Alena Wu.

6/28/2010

Made a 500 mL batch of LB broth

• When pouring in distilled water, pour a few mL at a time to avoid clumping.
• It takes 20g of powder to make 1 L of broth

Sterilized broth using the autoclave

• The temperature setting on the autoclave is off by a little bit
• Set dial 2 notches below 134°C.

6/29/2010

Started growing E. coli K12 cultures

• Poured 2 mL of LB broth into a Falcon tube
• Used strain of K12 from Dr. Lin's freezer
• Placed in incubator shaker for 24 hrs.

Added and inventoried supplies from Dr. Pinto's lab.

• Regular trash can be thrown out by going down one floor, then going outside to the trash bins.
• Chemical wastes must be cleaned by calling OSEH.

6/30/2010

Cryopreserved stock of K12

• 1 mL located in the iGEM box in the Lin lab.
• 1 mL located in the lab freezer.

7/1/2010

Cryopreserved DH5α according to protocol procedure on 6/30/2010

• Put 1 stock in -20°C fridge in 1239
• Put the other stock in the -80°C fridge in the Lin lab

7/7/2010

Obtain genomic DNA of CFT073 E. coli strain from Dr. Mobley's Lab

• stored in -20°C fridge on ice