Team:Michigan/Pili Expression
From 2010.igem.org
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==9/8/2010== | ==9/8/2010== | ||
- | Created frozen stocks of K12 and ΔFim::kan from overnight cultures. Stored them in box 1 in the ERB -20C freezer. | + | Created frozen stocks of K12 and ΔFim::kan w/the plasmid from overnight cultures. Stored them in box 1 in the ERB -20C freezer. |
Ran miniprep of K12 and ΔFim::kan, using 5 ml of each culture. | Ran miniprep of K12 and ΔFim::kan, using 5 ml of each culture. | ||
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Stored the product from miniprep in box 2 in the ERB -20C freezer. | Stored the product from miniprep in box 2 in the ERB -20C freezer. | ||
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+ | ==9/9/2010== | ||
+ | |||
+ | Cryopreserved frozen stocks of K12 and ΔFim::kan w/the plasmid. | ||
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+ | Ran digest to determine whether the plasmid and FimB were actually in the cell. Used protocol from previous digest on 8/14. | ||
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Revision as of 21:31, 10 September 2010