Team:Michigan/Pili Expression
From 2010.igem.org
(Difference between revisions)
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Electroporation of pBAD+FimB into K12 and ΔFimB::kan cells. | Electroporation of pBAD+FimB into K12 and ΔFimB::kan cells. | ||
- | <u>15 plates</u> | + | <u>15 plates</u> <br> |
- | 1x K12 control (amp) | + | 1x K12 control (amp) <br> |
- | 1x Plasmid control (amp + kan) | + | 1x Plasmid control (amp + kan) <br> |
- | 1x ΔFimB | + | 1x ΔFimB control (amp + kan) <br> |
- | 6x ΔFimB (amp + kan) | + | 6x ΔFimB (amp + kan) <br> |
- | 6x K12 (amp) | + | 6x K12 (amp) <br> |
+ | {| style="color:#1c2bf2;background-color:#fafa19;font-size:9pt;text-align:center" cellpadding="5" cellspacing="0" border="1" bordercolor="#fff" width="33%" | ||
+ | |- | ||
+ | ! Cell Type | ||
+ | ! Time Constant | ||
+ | |- | ||
+ | | Plasmid Control | ||
+ | | 5.6 | ||
+ | |- | ||
+ | | ΔFimB Control | ||
+ | | 5.4 | ||
+ | |- | ||
+ | | K12 Control | ||
+ | | 5.6 | ||
+ | |- | ||
+ | | ΔFimB A | ||
+ | | 5.4 | ||
+ | |- | ||
+ | | ΔFimB B | ||
+ | | 5.4 | ||
+ | |- | ||
+ | | K12 A | ||
+ | | 5.8 | ||
+ | |- | ||
+ | | K12 B | ||
+ | | 5.6 | ||
+ | |} | ||
+ | |||
+ | | | ||
=='''In the Lab'''== | =='''In the Lab'''== |
Revision as of 20:10, 3 September 2010