Team:Michigan/Pili Expression
From 2010.igem.org
(Difference between revisions)
MarcSinger (Talk | contribs) (→8/12/2010) |
|||
Line 161: | Line 161: | ||
Kevin, Marc, Alena | Kevin, Marc, Alena | ||
- | Met and discussed protocols for digestion and ligation | + | Met and discussed protocols for digestion and ligation of FIMB into pBAD. |
1. Added 5 mL of LB broth each to 2 50 mL falcon tubes in the ERB lab using sterile technique. | 1. Added 5 mL of LB broth each to 2 50 mL falcon tubes in the ERB lab using sterile technique. | ||
Line 175: | Line 175: | ||
5. At 8:05PM placed the two falcon tubes from step 4 into the incubator/shaker at 30C. | 5. At 8:05PM placed the two falcon tubes from step 4 into the incubator/shaker at 30C. | ||
+ | | | ||
=='''In the Lab'''== | =='''In the Lab'''== | ||
- | |||
[[Image:pili01.png|middle|250px]] | [[Image:pili01.png|middle|250px]] | ||
Revision as of 02:46, 13 August 2010