Team:Michigan/Pili Expression
From 2010.igem.org
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4 out of the 5 PCR reactions worked well according to the gel. | 4 out of the 5 PCR reactions worked well according to the gel. | ||
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+ | The 5th well could have been a loading problem or there wasn't enough DNA. | ||
[[media:Lab11.pdf|8/9/10 notes]] | [[media:Lab11.pdf|8/9/10 notes]] | ||
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==8/12/2010== | ==8/12/2010== | ||
- | Kevin, Marc, Alena | + | ''Kevin, Marc, Alena'' |
Met and discussed protocols for digestion and ligation of FIMB into pBAD. | Met and discussed protocols for digestion and ligation of FIMB into pBAD. | ||
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5. At 8:05PM placed the two falcon tubes from step 4 into the incubator/shaker at 30C. | 5. At 8:05PM placed the two falcon tubes from step 4 into the incubator/shaker at 30C. | ||
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+ | ==8/14/2010== | ||
+ | ''Kevin, Marc, Alena'' | ||
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+ | ===fimB PCR product Purification=== | ||
+ | #Used sample A and B of PCR product (save C and D for later) | ||
+ | ##total volume | ||
+ | (continue) | ||
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Revision as of 17:39, 15 August 2010