http://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&feed=atom&action=historyTeam:Michigan/Oil Sands October - Revision history2024-03-28T20:42:40ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&diff=170261&oldid=prevIfaulknr: /* 10/25/2010 */2010-10-27T01:08:03Z<p><span class="autocomment">10/25/2010</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ligation:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ligation:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*pSB1C3- 4.572 fmol/uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*pSB1C3 - 4.572 fmol/uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*VP130- 8.065 fmol/uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*VP130 - 8.065 fmol/uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*FimB- 24.2 fmol/uL</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*FimB - 24.2 fmol/uL</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>VP130 Ligation Mix:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>VP130 Ligation Mix:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Incubation:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Incubation:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*10 mins @ 22.<del class="diffchange diffchange-inline">5C</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*10 mins @ 22.<ins class="diffchange diffchange-inline">5ºC</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*10 mins @ <del class="diffchange diffchange-inline">65C</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*10 mins @ <ins class="diffchange diffchange-inline">65ºC</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ligations were transformed according to [[Media:Direct Plating Transformation Protocol.pdf|Direct Plating Transformation Protocol]].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ligations were transformed according to [[Media:Direct Plating Transformation Protocol.pdf|Direct Plating Transformation Protocol]].</div></td></tr>
</table>Ifaulknrhttp://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&diff=170245&oldid=prevIfaulknr: /* 10/24/2010 */2010-10-27T01:07:19Z<p><span class="autocomment">10/24/2010</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*16.7 uL UP H2O</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*16.7 uL UP H2O</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We then did the following transformations using the [[Media:Direct Plating Transformation Protocol.pdf|Direct Plating Transformation Protocol]] and the <del class="diffchange diffchange-inline">DH5alpha </del>comp cells Nick made using his [[Media:Quick Transformation of E. coli Competant Cell Preperation.pdf| Competent Cell Preparation for Quick Transformation of E. coli]] protocol:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We then did the following transformations using the [[Media:Direct Plating Transformation Protocol.pdf|Direct Plating Transformation Protocol]] and the <ins class="diffchange diffchange-inline">DH5α </ins>comp cells Nick made using his [[Media:Quick Transformation of E. coli Competant Cell Preperation.pdf| Competent Cell Preparation for Quick Transformation of E. coli]] protocol:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transformation (Plate Resistance):</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transformation (Plate Resistance):</div></td></tr>
</table>Ifaulknrhttp://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&diff=170229&oldid=prevIfaulknr: /* 10/23/2010 */2010-10-27T01:06:27Z<p><span class="autocomment">10/23/2010</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*FimB - 638 bp</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*FimB - 638 bp</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*VP130 - 2376, 1925 bp</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*VP130 - 2376, 1925 bp</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*pSB1AT3 -Flu- 6770, 15 bp</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*pSB1AT3-Flu - 6770, 15 bp</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The digests appear to have worked, although the ligations seem to have been incomplete (Roche Rapid Dephos & Ligation Kit).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The digests appear to have worked, although the ligations seem to have been incomplete (Roche Rapid Dephos & Ligation Kit).</div></td></tr>
</table>Ifaulknrhttp://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&diff=170224&oldid=prevIfaulknr: /* 10/23/2010 */2010-10-27T01:05:56Z<p><span class="autocomment">10/23/2010</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Expected Digest bands'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Expected Digest bands'''</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*FimB- 638 bp</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*FimB - 638 bp</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*VP130- 2376, 1925 bp</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*VP130 - 2376, 1925 bp</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*pSB1AT3-Flu- 6770, 15 bp</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*pSB1AT3 -Flu- 6770, 15 bp</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The digests appear to have worked, although the ligations seem to have been incomplete (Roche Rapid Dephos & Ligation Kit).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The digests appear to have worked, although the ligations seem to have been incomplete (Roche Rapid Dephos & Ligation Kit).</div></td></tr>
</table>Ifaulknrhttp://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&diff=170217&oldid=prevIfaulknr: /* 10/21/2010 */2010-10-27T01:05:40Z<p><span class="autocomment">10/21/2010</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**VF2- 33.9 nmol/.05 nmol/uL= 678 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**VF2- 33.9 nmol/.05 nmol/uL= 678 uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**VR- 40 nmol/ .05 nmol/uL= 800 uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**VR- 40 nmol/ .05 nmol/uL= 800 uL</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Used a 2 min <del class="diffchange diffchange-inline">extend</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Used a 2 min <ins class="diffchange diffchange-inline">extension</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Also, put the Cm and Tet plates from Tuesday in the <del class="diffchange diffchange-inline">4C</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Also, put the Cm and Tet plates from Tuesday in the <ins class="diffchange diffchange-inline">4ºC</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/23/2010 ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/23/2010 ==</div></td></tr>
</table>Ifaulknrhttp://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&diff=170207&oldid=prevIfaulknr: /* 10/19/2010 */2010-10-27T01:05:15Z<p><span class="autocomment">10/19/2010</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Marcus''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Marcus''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Made 10 Tet and 10 Cm plates. Also, Nick and I transformed DH5alpha comp cells from -<del class="diffchange diffchange-inline">80 </del>iGEM box with extra pSB1AT3-J23100-Flu ligation Ben and Kevin made yesterday. Transformed according to [[Media:Direct Plating Transformation Protocol.pdf|Direct Plating Transformation Protocol]]. We also transformed ''P. putida'' and ''P. fluorescens'' with pSB1AT3 as a test. No dilutions were made. It was noted while plating the cells that the ''Pseudomonas'' strains somehow wound up with less than 50 uL of cells. And due to the cells flocculating during storage, the ''Pseudomonas'' strains also had be vortexed to resuspend the cells.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Made 10 Tet and 10 Cm plates. Also, Nick and I transformed DH5alpha comp cells from -<ins class="diffchange diffchange-inline">80ºC </ins>iGEM box with extra pSB1AT3-J23100-Flu ligation Ben and Kevin made yesterday. Transformed according to [[Media:Direct Plating Transformation Protocol.pdf|Direct Plating Transformation Protocol]]. We also transformed ''P. putida'' and ''P. fluorescens'' with pSB1AT3 as a test. No dilutions were made. It was noted while plating the cells that the ''Pseudomonas'' strains somehow wound up with less than 50 uL of cells. And due to the cells flocculating during storage, the ''Pseudomonas'' strains also had be vortexed to resuspend the cells.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/21/2010 ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/21/2010 ==</div></td></tr>
</table>Ifaulknrhttp://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&diff=170197&oldid=prevIfaulknr: /* 10/17/2010 */2010-10-27T01:04:37Z<p><span class="autocomment">10/17/2010</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Marcus and Ben''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Marcus and Ben''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Nanodropped Nick's miniprep of pSB1AT3-Flu (#8) from yesterday, but found low concentrations of about 13-18 ng/uL since each mL of overnight was miniprepped individually. The samples were pooled by using a repurification protocol in the miniprep kit, eluted in Ultrapure water, and recived a final concentration of 40.2 ng/uL. The sequencing core requires 50 ng/uL, so a 5 mL overnight of pSB1AT3-Flu was made for Ben and Nick to miniprep tomorrow and a 2 mL overnight of <del class="diffchange diffchange-inline">DH5alpha </del>for comp cells.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Nanodropped Nick's miniprep of pSB1AT3-Flu (#8) from yesterday, but found low concentrations of about 13-18 ng/uL since each mL of overnight was miniprepped individually. The samples were pooled by using a repurification protocol in the miniprep kit, eluted in Ultrapure water, and recived a final concentration of 40.2 ng/uL. The sequencing core requires 50 ng/uL, so a 5 mL overnight of pSB1AT3-Flu was made for Ben and Nick to miniprep tomorrow and a 2 mL overnight of <ins class="diffchange diffchange-inline">DH5α </ins>for comp cells.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/18/2010 ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/18/2010 ==</div></td></tr>
</table>Ifaulknrhttp://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&diff=170182&oldid=prevIfaulknr: /* 10/16/2010 */2010-10-27T01:03:46Z<p><span class="autocomment">10/16/2010</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ben started the overnight cultures and assisted in OD measurement and initial centrifugation steps.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ben started the overnight cultures and assisted in OD measurement and initial centrifugation steps.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A colony of P. putida and P. fluorescence was picked from LB agar plates (10/6/10) and grown up in an overnight culture in 2ml Amp at <del class="diffchange diffchange-inline">30 C</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A colony of P. putida and P. fluorescence was picked from LB agar plates (10/6/10) and grown up in an overnight culture in 2ml Amp at <ins class="diffchange diffchange-inline">30ºC</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The overnight culture was added to 100 ml of LB media and grown at <del class="diffchange diffchange-inline">30C </del>for 4 hours. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The overnight culture was added to 100 ml of LB media and grown at <ins class="diffchange diffchange-inline">30ºC </ins>for 4 hours. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Protocol was carried out approximately 20 minutes after the following OD600 was recorded:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Protocol was carried out approximately 20 minutes after the following OD600 was recorded:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>P. Fluorescence – 0.610</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>P. Fluorescence – 0.610</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>One centrifuge tube containing p. putida culture failed during centrifugation at 5000 rpm. The protocol was altered by reducing the speed to 2500 rpm after this point to reduce the chance of failure. The cells are stored in the <del class="diffchange diffchange-inline">4C </del>fridge in room 1239.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>One centrifuge tube containing p. putida culture failed during centrifugation at 5000 rpm. The protocol was altered by reducing the speed to 2500 rpm after this point to reduce the chance of failure. The cells are stored in the <ins class="diffchange diffchange-inline">4ºC </ins>fridge in room 1239.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/17/2010 == </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/17/2010 == </div></td></tr>
</table>Ifaulknrhttp://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&diff=170168&oldid=prevIfaulknr: /* 10/15/2010 */2010-10-27T01:03:01Z<p><span class="autocomment">10/15/2010</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Incubation:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Incubation:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*<del class="diffchange diffchange-inline">37C </del>for 15 min</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">37ºC </ins>for 15 min</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*<del class="diffchange diffchange-inline">80C </del>for 20 min</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*<ins class="diffchange diffchange-inline">80ºC </ins>for 20 min</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ligated according [http://www.roche-applied-science.com/pack-insert/4898117a.pdf Roche Rapid Ligation Protocol].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ligated according [http://www.roche-applied-science.com/pack-insert/4898117a.pdf Roche Rapid Ligation Protocol].</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transformed according to [[Media:Direct Plating Transformation Protocol.pdf|Direct Plating Transformation Protocol]], but made 1:1 and 1:3 dilutions by adding 50 uL of cold, sterile DI water to the comp cells and mixing before each plating.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Transformed according to [[Media:Direct Plating Transformation Protocol.pdf|Direct Plating Transformation Protocol]], but made 1:1 and 1:3 dilutions by adding 50 uL of cold, sterile DI water to the comp cells and mixing before each plating.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/16/2010 ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/16/2010 ==</div></td></tr>
</table>Ifaulknrhttp://2010.igem.org/wiki/index.php?title=Team:Michigan/Oil_Sands_October&diff=170158&oldid=prevIfaulknr: /* 10/14/2010 */2010-10-27T01:02:16Z<p><span class="autocomment">10/14/2010</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Marcus''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Marcus''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Overnights made were only 2 mL, and should've been 5 mL. So 3 mL of fresh LB (with antibiotics) was added to 50 mL tubes, and the cultures were added to the fresh LB at 1 pm. Minipreps were made according to the [[Media:MODIFIED_Miniprep_Protocol.pdf|MODIFIED Miniprep]] at room temperature and stored in the <del class="diffchange diffchange-inline">4C</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Overnights made were only 2 mL, and should've been 5 mL. So 3 mL of fresh LB (with antibiotics) was added to 50 mL tubes, and the cultures were added to the fresh LB at 1 pm. Minipreps were made according to the [[Media:MODIFIED_Miniprep_Protocol.pdf|MODIFIED Miniprep]] at room temperature and stored in the <ins class="diffchange diffchange-inline">4ºC</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/15/2010 == </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== 10/15/2010 == </div></td></tr>
</table>Ifaulknr