Team:Michigan/Oil Sands October
From 2010.igem.org
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PCR Screening: | PCR Screening: | ||
- | 8 colonies were picked from each transformation plate except for VP130 | + | 8 colonies were picked from each transformation plate except for VP130 and suspended in 50 uL of water on a microplate. |
*Plate Layout | *Plate Layout | ||
Line 164: | Line 164: | ||
**Row 4- J61100 | **Row 4- J61100 | ||
- | The first two rows (Flu 1:1 and 1:3) were PCR'd by adding 1 uL of each sample to a PCR tube with a drop of mineral oil in them. A master mix was made | + | The first two rows (Flu 1:1 and 1:3) were PCR'd by adding 1 uL of each sample to a PCR tube with a drop of mineral oil in them. A master mix was made and 50 uL was added to each tube (should have been 49 uL). |
Master Mix (18x) | Master Mix (18x) |
Revision as of 04:08, 26 October 2010