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From 2010.igem.org

	Sunday	Monday	Tuesday	Wednesday	Thursday	Friday	Saturday
Week 1	-	6/28/2010	6/29/2010	6/30/2010	7/1/2010	7/2/2010	-
Week 2	-	-	-	7/8/2010	7/9/2010	7/10/2010	-	Week 3	-	-	-	-	-	-	-

Oil Sands Lab Notebook

This team includes Ann Lesnefsky,Bryce Rajabian, and Kilho Lee.

6/28/2010

Pseudomonas putida KT2440 Antibiotic Resistance

• Protocol for starting overnight culture from -80°C freezer
• All protocols can also be found in the Protocols section of the Notebook

6/29/2010

Pseudomonas putida KT2440 Antibiotic Resistance

• Protocol for making frozen stocks

6/29/2010 and 6/30/2010

Pseudomonas putida KT2440 Antibiotic Resistance

• Protocol for P. putida KT2440 antibotic resistance

7/1/2010

Literature Review Napthenic Acids Composition & HPLC Analysis

• Literature Review of the composition of napthenic acids in oil sands

7/2/2010

• Literature Review of HPLC columns for analysis of napthenic acids

Pseudomonas putida KT2440 Antibiotic Resistance

• Results

7/7/2010

Ann

Biobrick Transformation with Alex and Jennifer

• Made CaCl2 solution, and 100 mg/mL amphicilin stock solution according to the media section of the wiki
• Started an overnight culture of DH5alpha according to the heat shock transformation protocol
  • A 12 mL culture was started because we were multiplying the protocol by 4

7/8/2010

Ann

Biobrick Transformation with Marc, Katie and Audra according to the heat shock transformation protocol

• Started culture for biobrick transformation from over night at 2:30