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Team:Michigan/Media Recipes
From 2010.igem.org
(Difference between revisions)
| Line 21: | Line 21: | ||
''autoclave to sterilize'' | ''autoclave to sterilize'' | ||
| - | ''add antibiotics after autoclaving when warm to touch (about | + | ''add antibiotics after autoclaving when warm to touch (about 50°C)'' |
| - | ''pour plates when warm to the touch (about | + | ''pour plates when warm to the touch (about 50°C)'' |
'''Kanamycin Antibiotic Stock Solution (50 mg/mL)''' | '''Kanamycin Antibiotic Stock Solution (50 mg/mL)''' | ||
*250 mg of kanamycin | *250 mg of kanamycin | ||
*5 mL of DI water | *5 mL of DI water | ||
| - | ''filter sterilize and make 1 mL aliquots to store in the - | + | ''filter sterilize and make 1 mL aliquots to store in the -20°C freezer'' |
'''Tetracycline Antibiotic Stock Solution (50 mg/mL)''' | '''Tetracycline Antibiotic Stock Solution (50 mg/mL)''' | ||
| Line 34: | Line 34: | ||
*2.5 mL of DI water | *2.5 mL of DI water | ||
*2.5 mL of ethanol | *2.5 mL of ethanol | ||
| - | ''filter sterilize and make 1 mL aliquots to store in the - | + | ''filter sterilize and make 1 mL aliquots to store in the -20°C freezer'' |
Revision as of 22:30, 6 July 2010
Media Recipes
LB media
- 10 g LB broth
- 500 mL DI water
autoclave to sterilize
LB media with agar
- 10 g LB broth
- 7.5 g agar
- 500 mL DI water
autoclave to sterilize
add antibiotics after autoclaving when warm to touch (about 50°C)
pour plates when warm to the touch (about 50°C)
Kanamycin Antibiotic Stock Solution (50 mg/mL)
- 250 mg of kanamycin
- 5 mL of DI water
filter sterilize and make 1 mL aliquots to store in the -20°C freezer
Tetracycline Antibiotic Stock Solution (50 mg/mL)
- 250 mg of tetracycline
- 2.5 mL of DI water
- 2.5 mL of ethanol
filter sterilize and make 1 mL aliquots to store in the -20°C freezer
