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Revision as of 22:30, 6 July 2010 (view source) Kevijose (Talk | contribs) ← Older edit		Revision as of 16:55, 7 July 2010 (view source) Annlesn (Talk | contribs) Newer edit →
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	''pour plates when warm to the touch (about 50°C)''		''pour plates when warm to the touch (about 50°C)''
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		+	'''Ampicilin Antibiotic Stock Solution ()'''
	'''Kanamycin Antibiotic Stock Solution (50 mg/mL)'''		'''Kanamycin Antibiotic Stock Solution (50 mg/mL)'''

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Revision as of 16:55, 7 July 2010

Media Recipes

LB media

• 10 g LB broth
• 500 mL DI water

autoclave to sterilize

LB media with agar

• 10 g LB broth
• 7.5 g agar
• 500 mL DI water

autoclave to sterilize

add antibiotics after autoclaving when warm to touch (about 50°C)

pour plates when warm to the touch (about 50°C)

Ampicilin Antibiotic Stock Solution ()

Kanamycin Antibiotic Stock Solution (50 mg/mL)

• 250 mg of kanamycin
• 5 mL of DI water

filter sterilize and make 1 mL aliquots to store in the -20°C freezer

Tetracycline Antibiotic Stock Solution (50 mg/mL)

• 250 mg of tetracycline
• 2.5 mL of DI water
• 2.5 mL of ethanol

filter sterilize and make 1 mL aliquots to store in the -20°C freezer