Team:Michigan/Media Recipes

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Revision as of 19:58, 3 August 2010

Media Recipes

LB media

10 g LB broth
500 mL DI water

autoclave to sterilize

LB media with agar

10 g LB broth
7.5 g agar
500 mL DI water

autoclave to sterilize

add antibiotics after autoclaving when warm to touch (about 50°C)

pour plates when warm to the touch (about 50°C)

0.1 M CaCl2 solution

0.735 g of CaCl2*2 H20
50 mL of DI water

filter sterilize

1.0 M CaCl2 solution

7.35 g of CaCl2*2 H20
50 mL of DI water

filter sterilize

1.0 M MgSO4

12.323 g of MgSO4*7 H20
50 mL of DI water

filter sterilize

Ampicillin Antibiotic Stock Solution (100 mg/mL)

1 g of ampicillin
5 mL of DI water
5 mL of ethanol

filter sterilize and make 1 mL aliquotes to store in the -20°C freezer

Kanamycin Antibiotic Stock Solution (50 mg/mL)

250 mg of kanamycin
5 mL of DI water

filter sterilize and make 1 mL aliquots to store in the -20°C freezer

Tetracycline Antibiotic Stock Solution (50 mg/mL)

250 mg of tetracycline
2.5 mL of DI water
2.5 mL of ethanol

filter sterilize and make 1 mL aliquots to store in the -20°C freezer

IPTG stock solution (1 M)

0.25 g of IPTG
1049 uL of DI water

filter sterilize with .22 um filter

Cyclohexanecarboxylic acid

Cyclohexanecarboxylic acid is volatile should only be worked with in a hood. For accurate measurement use covered scale with sliding doors into the hood. Stored in the corrosive cabinet

0.075 g cyclohexanecarboxylic acid
50 mL of DI water

filter sterilize

@@ Line 63: / Line 63: @@
 '''Cyclohexanecarboxylic acid'''
+Cyclohexanecarboxylic acid is volatile should only be worked with in a hood.  For accurate measurement use covered scale with sliding doors into the hood. Stored in the corrosive cabinet
 *0.075 g cyclohexanecarboxylic acid
 *50 mL of DI water
 ''filter sterilize''