Team:Michigan/Kevin's Notebook

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6/28/2010

Made a 500 mL batch of LB broth

  • When pouring in distilled water, pour a few mL at a time to avoid clumping.
  • It takes 20g of powder to make 1 L of broth

Sterilized broth using the autoclave

  • The temperature setting on the autoclave is off by a little bit
  • Set dial 2 notches below 134 °C.

6/29/2010

Started growing E. coli K12 cultures

  • Poured 2 mL of LB broth into a Falcon tube
  • Used strain of K12 from Dr. Lin's freezer
  • Placed in incubator shaker for 24 hrs.

Added and inventoried supplies from Dr. Pinto's lab.

  • Regular trash can be thrown out by going down one floor, then going outside to the trash bins.
  • Chemical wastes must be cleaned by calling OSEH.

7/14/10

Poured agar plates. Created 500 mL of agar, poured 20 plates.

7/15/10

Performed digest on certain biobrick parts. Performed gel electrophoresis to determine validity of parts.

  • Most of the parts were the expected length.