Team:Michigan/Kevin's Notebook

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6/28/2010

Made a 500 mL batch of LB broth

  • When pouring in distilled water, pour a few mL at a time to avoid clumping.
  • It takes 20g of powder to make 1 L of broth

Sterilized broth using the autoclave

  • The temperature setting on the autoclave is off by a little bit
  • Set dial 2 notches below 134 °C.