Team:Michigan/Kevin's Notebook
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(Difference between revisions)
(New page: {{Michigan Header}} ==6/28/2010== Made a 500 mL batch of LB broth *When pouring in distilled water, pour a few mL at a time to avoid clumping. *It takes 20g of powder to make 1 L of broth...) |
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*The temperature setting on the autoclave is off by a little bit | *The temperature setting on the autoclave is off by a little bit | ||
*Set dial 2 notches below 134 °C. | *Set dial 2 notches below 134 °C. | ||
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+ | ==6/29/2010== | ||
+ | Started growing E. coli K12 cultures | ||
+ | *Poured 2 mL of LB broth into a Falcon tube | ||
+ | *Used strain of K12 from Dr. Lin's freezer | ||
+ | *Placed in incubator shaker for 24 hrs. | ||
+ | |||
+ | Added and inventoried supplies from Dr. Pinto's lab. | ||
+ | *Regular trash can be thrown out by going down one floor, then going outside to the trash bins. | ||
+ | *Chemical wastes must be cleaned by calling OSEH. |
Revision as of 21:36, 29 June 2010