Team:MIT phage background
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M13 bacteriophage is a long, filamentous virus that infects bacteria via interactions with the F-pilus. Below is a list of genes: | M13 bacteriophage is a long, filamentous virus that infects bacteria via interactions with the F-pilus. Below is a list of genes: | ||
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PIII/PVI (pointed end) – mediate termination of assembly and particle release from the membrane. About five copies.</li> | PIII/PVI (pointed end) – mediate termination of assembly and particle release from the membrane. About five copies.</li> | ||
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PV – dimerizes and binds ssDNA, packaging it into a rod for assembly</li></ul> | PV – dimerizes and binds ssDNA, packaging it into a rod for assembly</li></ul> | ||
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<b>M13 ASSEMBLY</b> | <b>M13 ASSEMBLY</b> |
Revision as of 00:50, 25 October 2010
hairy cells and polymerizing phage - background |
M13 ANATOMY M13 bacteriophage is a long, filamentous virus that infects bacteria via interactions with the F-pilus. Below is a list of genes:
M13 ASSEMBLY
PHAGE DISPLAY By genetically modifying the phage, fusion proteins can be created that become integrated into the phage coat in a defined manner. Usually these new proteins are linked to the pIII or pVIII coat proteins (though PVII or PIX can be used). Thus, novel proteins can be displayed on the phage coat. This has historically been used as a mechanism for screening libraries of proteins or peptides (e.g., antibodies) for binding to a specific substrate or ligand of interest. In a process called panning, a population of phage is iteratively enriched for those that bind a substrate. Phage with proteins that do not bind are washed away and the remaining phage can then be amplified through infection. HYPERPHAGE Hyperphage is a plasmid with the gene for pIII truncated. The pIII protein is required for phage exit from the host cell membrane; phage without a proper pIII grow into long fibril-like structures called polyphage. The goal is to crosslink these polyphage.
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