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Team:MIT mammalian Mechanosensation
From 2010.igem.org
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<table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">The Search for Mechanosensitive Promoters</div></td> | <table width=650px style="background-color: white; margin-top:5px; padding: 10px;"><tr><td><div class="bodybaby">The Search for Mechanosensitive Promoters</div></td> | ||
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| + | <div class="bodybaby" id="first">Overview</div><br> | ||
| + | <b class="bolded" id="overview">We set out to find a mechnosensitive promoter, one capable of acting as a sensor of applied pressure in our final system. A literature search revealed </b><br> | ||
| + | To begin, we searched the literature for well-characterized mechanosensitive promoters; we restricted our pool of candidates to promoters that had been shown, mostly by upregulation of a fluorescent marker, to be directly responsive to mechanical stress. The nature of the promoters varied. We included several short oligonucleotide sequences SRE/CRE2 and NR1/2, that had been shown to confer sensitivitiy to shear stress when placed in front of a ‘dummy’ SV40 promoter. Promoters for the genes PAI-2 and cfos were identified as mechanosensitive in the literature; we used portions of these promoters which were shown to be responsible for the mechanosensitivity. | ||
</td></table> | </td></table> | ||
Revision as of 21:27, 26 October 2010
iGEM 2010
The Search for Mechanosensitive Promoters |
|
Overview We set out to find a mechnosensitive promoter, one capable of acting as a sensor of applied pressure in our final system. A literature search revealed To begin, we searched the literature for well-characterized mechanosensitive promoters; we restricted our pool of candidates to promoters that had been shown, mostly by upregulation of a fluorescent marker, to be directly responsive to mechanical stress. The nature of the promoters varied. We included several short oligonucleotide sequences SRE/CRE2 and NR1/2, that had been shown to confer sensitivitiy to shear stress when placed in front of a ‘dummy’ SV40 promoter. Promoters for the genes PAI-2 and cfos were identified as mechanosensitive in the literature; we used portions of these promoters which were shown to be responsible for the mechanosensitivity. |
