http://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&feed=atom&action=historyTeam:MIT k415031 - Revision history2024-03-28T08:28:54ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&diff=172910&oldid=prevSupacalafrglstic at 03:46, 27 October 20102010-10-27T03:46:52Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><hr></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b class="bolded"> K415031 is less leaky than R0065 </b<del class="diffchange diffchange-inline">><hr</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b class="bolded"> K415031 is less leaky than R0065 </b></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://static.igem.org/mediawiki/2010/a/af/Leakiness.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/a/af/Leakiness.png"></a><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://static.igem.org/mediawiki/2010/a/af/Leakiness.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/a/af/Leakiness.png"></a><br></div></td></tr>
</table>Supacalafrglstichttp://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&diff=172904&oldid=prevSupacalafrglstic at 03:46, 27 October 20102010-10-27T03:46:30Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b class="bolded"> K415031 is less leaky than R0065 </b></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b class="bolded"> K415031 is less leaky than R0065 </b<ins class="diffchange diffchange-inline">><hr</ins>></div></td></tr>
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</table>Supacalafrglstichttp://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&diff=172898&oldid=prevSupacalafrglstic at 03:45, 27 October 20102010-10-27T03:45:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Slightly higher binding than <a href="https://2010.igem.org/Team:MIT_k415032">K415032</a>. <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Slightly higher binding than <a href="https://2010.igem.org/Team:MIT_k415032">K415032</a>. <br><br></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a<del class="diffchange diffchange-inline">></center</del>><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><b class="bolded"> K415031 is less leaky than R0065 </b></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a href="https://static.igem.org/mediawiki/2010/a/af/Leakiness.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/a/af/Leakiness.png"></a><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The above images are FACS scatter plots of co-transformations of either pTSMa (<a href="https://2010.igem.org/Team:MIT_k415300">BBa_K415300</a>) and <a href="https://2010.igem.org/Team:MIT_k415069">K415069</a> or pTSMa and <a href="https://2010.igem.org/Team:MIT_k415023">BBa_K415023</a>. The x-axis represents the amount of green fluorescence, and the y-axis represents the amount of red fluorescence. The parts K415069 and K415023 differ only in a single promoter sequence: K415069 has the K415031 promoter in place of a single R0065 promoter in the K415023 sequence. In this way, the more "leaky" the promoter, the more red fluorescing cells.<br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">As you can see in the images, fewer cells with pTSMa+K415069 are leaky: 3% of total cells with pTSMa+K415069 fluoresce red, and 15% of total cells with pTSMa+K415023 fluoresce red.</center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:MIT_k415301"><b>&larr; K415301</b></a><a style="float:right;" href="https://2010.igem.org/Team:MIT_k415032"><b>K415032 &rarr;</b></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:MIT_k415301"><b>&larr; K415301</b></a><a style="float:right;" href="https://2010.igem.org/Team:MIT_k415032"><b>K415032 &rarr;</b></a></div></td></tr>
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</table>Supacalafrglstichttp://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&diff=171914&oldid=prevSupacalafrglstic at 02:40, 27 October 20102010-10-27T02:40:39Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:<del class="diffchange diffchange-inline">MIT_original</del>"><b>&larr; <del class="diffchange diffchange-inline">Original Parts</del></b></a><a style="float:right;" href="https://2010.igem.org/Team:<del class="diffchange diffchange-inline">MIT_k415301</del>"><b><del class="diffchange diffchange-inline">K415301 </del>&rarr;</b></a></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:<ins class="diffchange diffchange-inline">MIT_k415301</ins>"><b>&larr; <ins class="diffchange diffchange-inline">K415301</ins></b></a><a style="float:right;" href="https://2010.igem.org/Team:<ins class="diffchange diffchange-inline">MIT_k415032</ins>"><b><ins class="diffchange diffchange-inline">K415032 </ins>&rarr;</b></a></div></td></tr>
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</table>Supacalafrglstichttp://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&diff=171907&oldid=prevSupacalafrglstic at 02:40, 27 October 20102010-10-27T02:40:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415031"><b>See the Parts Registry Page!! &rarr;</b></a><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415031"><b>See the Parts Registry Page!! &rarr;</b></a><br><br></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b class="bolded">Improved PLux/cI-OR Promoter</b><br>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b class="bolded">Improved PLux/cI-OR Promoter</b><br>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on<ins class="diffchange diffchange-inline">.<br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Slightly higher binding than <a href="https://2010.igem.org/Team:MIT_k415032">K415032</a></ins>. <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a></center><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox" title="Regulatory promoter pLux/cI-OR"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a></center><br><br></div></td></tr>
</table>Supacalafrglstichttp://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&diff=171469&oldid=prevSupacalafrglstic at 02:14, 27 October 20102010-10-27T02:14:52Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b class="bolded">Improved PLux/cI-OR Promoter</b><br>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b class="bolded">Improved PLux/cI-OR Promoter</b><br>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a></center><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox<ins class="diffchange diffchange-inline">" title="Regulatory promoter pLux/cI-OR</ins>"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a></center><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:MIT_original"><b>&larr; Original Parts</b></a><a style="float:right;" href="https://2010.igem.org/Team:MIT_k415301"><b>K415301 &rarr;</b></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:MIT_original"><b>&larr; Original Parts</b></a><a style="float:right;" href="https://2010.igem.org/Team:MIT_k415301"><b>K415301 &rarr;</b></a></div></td></tr>
</table>Supacalafrglstichttp://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&diff=171454&oldid=prevSupacalafrglstic at 02:14, 27 October 20102010-10-27T02:14:14Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:14, 27 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b class="bolded">Improved PLux/cI-OR Promoter</b><br>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b class="bolded">Improved PLux/cI-OR Promoter</b><br>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><center></ins><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a<ins class="diffchange diffchange-inline">></center</ins>><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:MIT_original"><b>&larr; Original Parts</b></a><a style="float:right;" href="https://2010.igem.org/Team:MIT_k415301"><b>K415301 &rarr;</b></a></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://2010.igem.org/Team:MIT_original"><b>&larr; Original Parts</b></a><a style="float:right;" href="https://2010.igem.org/Team:MIT_k415301"><b>K415301 &rarr;</b></a></div></td></tr>
</table>Supacalafrglstichttp://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&diff=171448&oldid=prevSupacalafrglstic at 02:13, 27 October 20102010-10-27T02:13:58Z<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:13, 27 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415031"><b>See the Parts Registry Page!! &rarr;</b></a><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415031"><b>See the Parts Registry Page!! &rarr;</b></a><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b class="bolded">Improved PLux/cI-OR Promoter</b>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b class="bolded">Improved PLux/cI-OR Promoter</b<ins class="diffchange diffchange-inline">><br</ins>>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br></div></td></tr>
</table>Supacalafrglstichttp://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&diff=171444&oldid=prevSupacalafrglstic at 02:13, 27 October 20102010-10-27T02:13:49Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 02:13, 27 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415031"><b>See the Parts Registry Page!! &rarr;</b></a><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415031"><b>See the Parts Registry Page!! &rarr;</b></a><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b class="bolded">Improved PLux/cI-OR Promoter</b></ins>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br></div></td></tr>
</table>Supacalafrglstichttp://2010.igem.org/wiki/index.php?title=Team:MIT_k415031&diff=171429&oldid=prevSupacalafrglstic: New page: {{CM_css}} <html> <head> <style> #topnav li.notebook a { background-color: #016b9d; } #topnav li.notebook ul { display: block; } #content { background-image: url('https://2010.igem.org...2010-10-27T02:13:19Z<p>New page: {{CM_css}} <html> <head> <style> #topnav li.notebook a { background-color: #016b9d; } #topnav li.notebook ul { display: block; } #content { background-image: url('https://2010.igem.org...</p>
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<li><a href="https://2010.igem.org/Team:MIT_parts">All Parts</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_composite">Composite</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_original">Original</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_backbones">Backbones</a></li><br />
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<li><a href="https://2010.igem.org/Team:MIT_gels">Gels</a></li><br />
<li><a href="https://2010.igem.org/Team:MIT_experimental">Experimental</a></li><br />
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<div class="bodybaby">K415031</div><br><br />
From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415031"><b>See the Parts Registry Page!! &rarr;</b></a><br><br><br />
<br />
This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br><br />
<br />
<a href="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png" class="thickbox"><img src="https://static.igem.org/mediawiki/2010/5/5a/Regulatory_yo.png"></a><br><br><br />
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<a href="https://2010.igem.org/Team:MIT_original"><b>&larr; Original Parts</b></a><a style="float:right;" href="https://2010.igem.org/Team:MIT_k415301"><b>K415301 &rarr;</b></a><br />
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