Team:LMU-Munich/ProSearch

From 2010.igem.org

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This system is to search for new enhancer-promoter combinations that efficiently trigger gene expression in response to different stimuli. This part of the project is carried out through a bioinformatic modeling process. The end-product is a databank of related genes, their promoters and transcription factors at the input of a wished environmental condition. This system is useful for the construction of new inducible systems trying to utilize apoptosis or other gene expression in the human cell-line 293T.
This system is to search for new enhancer-promoter combinations that efficiently trigger gene expression in response to different stimuli. This part of the project is carried out through a bioinformatic modeling process. The end-product is a databank of related genes, their promoters and transcription factors at the input of a wished environmental condition. This system is useful for the construction of new inducible systems trying to utilize apoptosis or other gene expression in the human cell-line 293T.
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http://igemlmu.scienceontheweb.net/form.html
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#!/bin/sh
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echo Content-type: text/html
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echo ""
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### $QUERY_STRING=namex=valuex&namey=valuey&namez=valuez
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QS=`echo $QUERY_STRING | sed -e s/\\\`//g -e s/\"//g`
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str1=`echo $QS|awk -F\& '{printf $1}'`
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str2=`echo $QS|awk -F\& '{printf $2}'`
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arg1=`echo $str1|awk -F= '{printf $2}'`
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arg2=`echo $str2|awk -F= '{printf $2}'`
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/bin/cat << EOM
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<HTML>
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<HEAD><TITLE>Transcription Factors of genes differentially expressed in condition $arg1 </TITLE>
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</HEAD>
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<BODY bgcolor="#cccccc" text="#000000">
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<HR SIZE=5>
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<H1>TFs of genes differentially expressed in condition $arg1</H1>
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<HR SIZE=5>
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<P>
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<SMALL>
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<PRE>
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EOM
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java GetTFsPromoters "$arg1" "$arg2"
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cat << EOM
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</PRE>
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</SMALL>
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<P>
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</BODY>
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</HTML>
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EOM
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Revision as of 20:50, 13 October 2010


ProSearch System

This system is to search for new enhancer-promoter combinations that efficiently trigger gene expression in response to different stimuli. This part of the project is carried out through a bioinformatic modeling process. The end-product is a databank of related genes, their promoters and transcription factors at the input of a wished environmental condition. This system is useful for the construction of new inducible systems trying to utilize apoptosis or other gene expression in the human cell-line 293T.

  1. !/bin/sh

echo Content-type: text/html echo ""

      1. $QUERY_STRING=namex=valuex&namey=valuey&namez=valuez

QS=`echo $QUERY_STRING | sed -e s/\\\`//g -e s/\"//g` str1=`echo $QS|awk -F\& '{printf $1}'` str2=`echo $QS|awk -F\& '{printf $2}'` arg1=`echo $str1|awk -F= '{printf $2}'` arg2=`echo $str2|awk -F= '{printf $2}'`

/bin/cat << EOM Transcription Factors of genes differentially expressed in condition $arg1


TFs of genes differentially expressed in condition $arg1


EOM

java GetTFsPromoters "$arg1" "$arg2"

cat << EOM

EOM