Team:LMU-Munich/Jump-or-die/Schedule/SequenzPCR8

From 2010.igem.org

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==Sequence PCR8==
==Sequence PCR8==
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verified by sequencing of vector BBa_K368019
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=== with forward primer ===
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NNNNNNNNNNNNNNNNNNNNNNTATNNNNNNNGCGTNNCNCGAGGCAGAATT
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NNNNTAAAAAAAATCCTTAGCTTTCGCTANNNNNNNNNNNNNNNNNNCGGCCG
 +
 +
CTTCTAGAGATGGCGATGCAANNTNTNNNGGGGCNAACATTCGACTTCCACCT
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GAAGTAAATCGGATATTGTATATAAGAAATTTGCCATACAAAATCACAGCTGA
 +
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ANAAATGTATGATATATTTGGGAAATATGGACCTATTCGTCAAATCAGAGTGGG
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 +
GAACACACCTGAAACTAGAGGAACAGCTTATGTGGTCTATGAGGACATCTTTGA
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 +
TGCCAAGAATGCATGTGATCACCTATCGGGATTCAATGTTTGTAACAGATACC
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 +
TTGTGGTTTTGTACTATAATGCCAACAGGGCATTTCAGAAGATGGACACAAAG
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AAGAAGGAGGAACAGTTGAAGCTTCTCAAGGNNANNNNTGGCATCAACACATA
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TCCTCCCAAGATGAAACATCACCNNCNCCANCNCCCCATGAGCGGCCTGGTGC
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CGCGCGGCAGCGCCATGGGAGAAAGCTTGTTTAAGGGACCACGTGATTACAAC
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CCGATATCGAGCACCATTTGTCATTTGACGAATGAATCTGATGGGCACACAACA
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TCGTTGTATGGTATTGGATTTGGTCCCNTCATCATTACAAACAAGCACNTTGTT
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TCNTCNCNATAATGGAACACTGTTGNTCCCNAT
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 +
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=== with reverse primer ===
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NNNNNNNNNNNNNNNNNNNNACCNGANCNNNGCGAGTCAGTGAGCGAGGAAGCCTG
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CATAACGCGAAGTAATCTTTTCGGTNNNTNGGNNGGGNAGGGTGGTGACACCTTGCCC
 +
 +
TTTTTTGCCGGNNNNAGCGGNCGCTACTAGTAGAATTGGAAGTACAAGTTTCCCCTT
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GCGAGTACACCAATTCATTCATGAGTTGAGTCGCTTCCTTAACTGGCTGAAAAGGTT
 +
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CTTCAGGTTTAACCATGAAAACTTTATGGCCCCCCCACAATACTGAGTCAGCGTTTA
 +
 +
ATCGCCAACCACTAACCCACTGCTGCGCCTCCTGATTTGTCAACAATTCCATGAAGT
 +
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TTTTCGGCACGCTTGTGAAATAATTGTTTGTGTTGGTGAAATTCGATGCTGAGTGTA
 +
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TACCAACAATGAACCCATCTCTAGTTGATACTAATGGACTGCCACACTGCNNATCCT
 +
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TGGTTTGAATCCAATGCTTCCAGAATATACCATCAGATGAAGGGAATGNNCNACTCGTGTC
 +
 +
TGACACCATGCTAGACATGCTCTTAGCTTGGAAGTTGGTTGTCACAAGACATATGCGCT
 +
 +
CTTCCCTTTGTGGCTCTCTAAATTTCNNCTTTTGAGGAAATGGTGGGAAATCCTTAGGCA
 +
 +
TGCGAATAATCATCATGTCCCTCCCATCAATGAGGTGTTGTTGCAAAGTCGTGGTGTCCTT
 +
 +
GACCTTGAATACACCATGTAGTGATTGGACCAACAGTGTTCCATTATTGCGACGAAANCNA
 +
 +
GTGCTTGTTTTGTAATGATGAAGGGACCAAATCCAATACCATACAACGATGTTGTGTGCC
 +
 +
CATCANANTCNTTCGTCAAATGACAAATGGTGCTCGATATCGGGTTGTAATCACGTGGTC
 +
 +
CCTTTAACAAGNTTTNTCCATGGCGCTGCCGCGCGGCACCNGGNCGCNCATGGNNGATGG
 +
 +
NTGATGGTGATGTTTCATCCTGGGAGGATNTGTTGTTNATGCCATNTTTCTCTTGANAAG
 +
 +
NTTCANNGTTCNNCTTCTTNTTTGTGTCNNTCTTCNAAATGCNTGTTGNNTTNNAGNNNA
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 +
AACNNCAANGG
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Latest revision as of 17:26, 24 October 2010


Sequence PCR8

verified by sequencing of vector BBa_K368019

with forward primer

NNNNNNNNNNNNNNNNNNNNNNTATNNNNNNNGCGTNNCNCGAGGCAGAATT

NNNNTAAAAAAAATCCTTAGCTTTCGCTANNNNNNNNNNNNNNNNNNCGGCCG

CTTCTAGAGATGGCGATGCAANNTNTNNNGGGGCNAACATTCGACTTCCACCT

GAAGTAAATCGGATATTGTATATAAGAAATTTGCCATACAAAATCACAGCTGA

ANAAATGTATGATATATTTGGGAAATATGGACCTATTCGTCAAATCAGAGTGGG

GAACACACCTGAAACTAGAGGAACAGCTTATGTGGTCTATGAGGACATCTTTGA

TGCCAAGAATGCATGTGATCACCTATCGGGATTCAATGTTTGTAACAGATACC

TTGTGGTTTTGTACTATAATGCCAACAGGGCATTTCAGAAGATGGACACAAAG

AAGAAGGAGGAACAGTTGAAGCTTCTCAAGGNNANNNNTGGCATCAACACATA

TCCTCCCAAGATGAAACATCACCNNCNCCANCNCCCCATGAGCGGCCTGGTGC

CGCGCGGCAGCGCCATGGGAGAAAGCTTGTTTAAGGGACCACGTGATTACAAC

CCGATATCGAGCACCATTTGTCATTTGACGAATGAATCTGATGGGCACACAACA

TCGTTGTATGGTATTGGATTTGGTCCCNTCATCATTACAAACAAGCACNTTGTT

TCNTCNCNATAATGGAACACTGTTGNTCCCNAT


with reverse primer

NNNNNNNNNNNNNNNNNNNNACCNGANCNNNGCGAGTCAGTGAGCGAGGAAGCCTG

CATAACGCGAAGTAATCTTTTCGGTNNNTNGGNNGGGNAGGGTGGTGACACCTTGCCC

TTTTTTGCCGGNNNNAGCGGNCGCTACTAGTAGAATTGGAAGTACAAGTTTCCCCTT

GCGAGTACACCAATTCATTCATGAGTTGAGTCGCTTCCTTAACTGGCTGAAAAGGTT

CTTCAGGTTTAACCATGAAAACTTTATGGCCCCCCCACAATACTGAGTCAGCGTTTA

ATCGCCAACCACTAACCCACTGCTGCGCCTCCTGATTTGTCAACAATTCCATGAAGT

TTTTCGGCACGCTTGTGAAATAATTGTTTGTGTTGGTGAAATTCGATGCTGAGTGTA

TACCAACAATGAACCCATCTCTAGTTGATACTAATGGACTGCCACACTGCNNATCCT

TGGTTTGAATCCAATGCTTCCAGAATATACCATCAGATGAAGGGAATGNNCNACTCGTGTC

TGACACCATGCTAGACATGCTCTTAGCTTGGAAGTTGGTTGTCACAAGACATATGCGCT

CTTCCCTTTGTGGCTCTCTAAATTTCNNCTTTTGAGGAAATGGTGGGAAATCCTTAGGCA

TGCGAATAATCATCATGTCCCTCCCATCAATGAGGTGTTGTTGCAAAGTCGTGGTGTCCTT

GACCTTGAATACACCATGTAGTGATTGGACCAACAGTGTTCCATTATTGCGACGAAANCNA

GTGCTTGTTTTGTAATGATGAAGGGACCAAATCCAATACCATACAACGATGTTGTGTGCC

CATCANANTCNTTCGTCAAATGACAAATGGTGCTCGATATCGGGTTGTAATCACGTGGTC

CCTTTAACAAGNTTTNTCCATGGCGCTGCCGCGCGGCACCNGGNCGCNCATGGNNGATGG

NTGATGGTGATGTTTCATCCTGGGAGGATNTGTTGTTNATGCCATNTTTCTCTTGANAAG

NTTCANNGTTCNNCTTCTTNTTTGTGTCNNTCTTCNAAATGCNTGTTGNNTTNNAGNNNA

AACNNCAANGG