Team:HokkaidoU Japan/Protocols

From 2010.igem.org

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{{Template:HokkaidoU_Japan}}
{{Template:HokkaidoU_Japan}}
=Protocols=
=Protocols=
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# Preparation of Competent cells (''E. coli'' DH5a)
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* Preparation of Competent cells (''E. coli'' DH5a)
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# Bacterial Transformations
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* Bacterial Transformations
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# Mini-prep (Alkaline SDS Method)
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* Mini-prep (Alkaline SDS Method)
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# PCR
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* PCR
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# Restriction Enzyme Digestions
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* Restriction Enzyme Digestions
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# Ligation
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* DNA ligation
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# Agarose gel electrophoresis
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* Agarose gel electrophoresis
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# Electroporation
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* Electroporation

Revision as of 14:59, 21 September 2010

Protocols

  • Preparation of Competent cells (E. coli DH5a)
  • Bacterial Transformations
  • Mini-prep (Alkaline SDS Method)
  • PCR
  • Restriction Enzyme Digestions
  • DNA ligation
  • Agarose gel electrophoresis
  • Electroporation