Team:HokkaidoU Japan/Protocols

From 2010.igem.org

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=Protocols=
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# Preparation of Competent cells (''E. coli'' DH5a)
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# Bacterial Transformations
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# Mini-prep (Alkaline SDS Method)
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# PCR
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# Restriction Enzyme Digestions
 +
# Ligation
 +
# Agarose gel electrophoresis
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# Electroporation

Revision as of 14:54, 21 September 2010

Protocols

  1. Preparation of Competent cells (E. coli DH5a)
  2. Bacterial Transformations
  3. Mini-prep (Alkaline SDS Method)
  4. PCR
  5. Restriction Enzyme Digestions
  6. Ligation
  7. Agarose gel electrophoresis
  8. Electroporation
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Protocols"